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Team:Paris Saclay/Notebook/July/12
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*design of oligo for amplifying AmiCP+RBS. | *design of oligo for amplifying AmiCP+RBS. | ||
For PCBs sensor system: | For PCBs sensor system: | ||
| - | *had the | + | *had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock. |
| - | + | *plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8. | |
=='''Lab work'''== | =='''Lab work'''== | ||
Revision as of 02:16, 22 September 2013
Notebook : July 12
summary
For fnr regulator system:
- performed 4 transformations(3 terminator and 1 RBS+LacZ+terminator into competent cells).
- design of oligo for amplifying AmiCP+RBS.
For PCBs sensor system:
- had the promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8 in stock.
- plasmid DNA extraction from promoter BphR1 clone 5 and 6, promoter BphR2 clone 3 and 4, promoter BphA1 clone 5 to 8.
Lab work
constructing
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