Latest revision as of 09:55, 30 September 2013

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Notebook : July 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017

Zhou

Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.

Protocol : Bacterial transformation

2 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.

B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2

Zhou

Used quantities :

Glycerol 85% : 425
Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BBa_K1155002 and BphR2

Anaïs

We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.

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@@ Line 1: / Line 1: @@
-<u>Transformation</u>
-<br>
-<p>We suspended the DNA with water from iGEM plate and performed several transformation.</p><br>
-*BBa_I732019: LacZ+RBS+terminator into plasmid BBa_I732950(ampicillin resitant).
-*BBa_B0015: 2 terminators BBa_B0010+BBa_B0012(4F plate 3 kit 2013) into plasmid PSB1C3.
-*BBa_B0017: 2 terminators BBa_B0010+BBa_B0010 into plasmid PSB1C3.
-*Simple terminator BBa_B0010 into plasmid PSB1A2.
 {{Team:Paris_Saclay/incl_debut_generique}}
@@ Line 28: / Line 5: @@
 =='''Lab work'''==
-==='''A - Aerobic/Anaerobic regulation system''===
+==='''A - Aerobic/Anaerobic regulation system'''===
-===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
+===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
-===='''1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017, Bba_B0010'''====
+===='''1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017'''====
 Zhou
@@ Line 38: / Line 15: @@
 {|
 | style="border:1px solid black;padding:5px;background-color:#DE;" |
-Transformation of 07/10/13 of Bba_B0010 and Bba_I732019 didn't works. We will do it again.
+Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.
 |}
-Protocol : [[Team:Paris_Saclay/Protocols/Bacterial transformation|Bacterial transformation]]
+Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
-===='''1 -Design of RBS-AmilCP oligos'''====
+===='''2 -Design of RBS-AmilCP oligos'''====
 Abdou, Anaïs
@@ Line 52: / Line 29: @@
 ==='''B - PCB sensor system'''===
-===='''Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2'''====
+===='''Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2'''====
-===='''1 - Stock of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2'''====
+===='''1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2'''====
 Zhou
@@ Line 62: / Line 39: @@
 * Culture : 1µL
-===='''2 - Extraction of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2 from DH5α'''====
+===='''2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α'''====
 Abdou, Sheng
@@ Line 68: / Line 45: @@
 Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
-===='''3 - Streak of BphA1 and BphR2'''====
+===='''3 - Streak of BBa_K1155002 and BphR2'''====
 Anaïs
-We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.
+We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.
 {| border="1" align="center"
@@ Line 79: / Line 57: @@
 |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
-|<big>Next week</big>
+|[[Team:Paris Saclay/Notebook/July/15|<big>Next week</big>]]
 |}
 {{Team:Paris_Saclay/incl_fin}}

Team:Paris Saclay/Notebook/July/12

From 2013.igem.org