Team:Paris Saclay/Notebook/July/12

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='''Notebook : July 12'''=
='''Notebook : July 12'''=
=='''Lab work'''==
=='''Lab work'''==
-
constructing
+
 
 +
==='''A - Aerobic/Anaerobic regulation system'''===
 +
 
 +
===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
 +
 
 +
===='''1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017'''====
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 +
Zhou
 +
 
 +
{|
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| style="border:1px solid black;padding:5px;background-color:#DE;" |
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Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.
 +
|}
 +
 
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Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
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 +
===='''2 -Design of RBS-AmilCP oligos'''====
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 +
Abdou, Anaïs
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We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
 +
 
 +
 
 +
==='''B - PCB sensor system'''===
 +
 
 +
===='''Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2'''====
 +
 
 +
===='''1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2'''====
 +
 
 +
Zhou
 +
 
 +
Used quantities :
 +
* Glycerol 85% : 425
 +
* Culture : 1µL
 +
 
 +
===='''2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α'''====
 +
 
 +
Abdou, Sheng
 +
 
 +
Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
 +
 
 +
===='''3 - Streak of BBa_K1155002 and BphR2'''====
 +
 
 +
Anaïs
 +
 
 +
We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.
 +
 
 +
 
 +
{| border="1" align="center"
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|[[Team:Paris Saclay/Notebook/July/11|<big>Previous day</big>]]
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|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
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|[[Team:Paris Saclay/Notebook/July/15|<big>Next week</big>]]
 +
|}
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 09:55, 30 September 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : July 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017

Zhou

Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.

Protocol : Bacterial transformation

2 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.


B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2

Zhou

Used quantities :

  • Glycerol 85% : 425
  • Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BBa_K1155002 and BphR2

Anaïs

We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.


Previous day Back to calendar Next week

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