Team:Paris Saclay/Notebook/July/15

From 2013.igem.org

(Difference between revisions)
Line 43: Line 43:
Protocol : [[Team:Paris_Saclay/Protocols/Ethanol precipitation|Ethanol precipitation]]
Protocol : [[Team:Paris_Saclay/Protocols/Ethanol precipitation|Ethanol precipitation]]
 +
We used 15µL of DNA.
 +
 +
===='''3 - PCR of digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3'''====
 +
 +
Anaïs, Sheng, Zhou
 +
 +
Used quantities :
 +
 +
....
 +
 +
PCR Program :
 +
 +
* BphR1 :
 +
 +
[[File:PsPCRR11507.jpg|400px]]
 +
 +
* BphA1, BphR2 :
 +
 +
[[File:PsPCRR2A11507.jpg|400px]]
{| border="1" align="center"
{| border="1" align="center"

Revision as of 14:43, 25 September 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : July 15

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 - Transformation of Bba_I732019 and Bba_B0010 in DH5α

B - PCB sensor system

Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2

1 - Digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments

Abdou

Used quantities :

  • EcoRI/PstI :
    • DNA : 8µL
    • Buffer Orange : 1.6µL
    • EcoRI : 1µL
    • PstI : 1µL
    • H2O : 4.4µL
  • SacII :
    • DNA : 8µL
    • Buffer Blue : 1.6µL
    • SacII : 1µL
    • H2O : 5.4µL

We let the digestion 1h30 at 37°C.

2 - Denaturation of EcoRI/PstI and SacII used for the digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3

Abdou

Protocol : Ethanol precipitation

We used 15µL of DNA.

3 - PCR of digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3

Anaïs, Sheng, Zhou

Used quantities :

....

PCR Program :

  • BphR1 :

PsPCRR11507.jpg

  • BphA1, BphR2 :

PsPCRR2A11507.jpg

Previous week Back to calendar next day

Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Notebook/July/15"