"
Team:Paris Saclay/Notebook/July/15
From 2013.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining Bba_K1155000'''==== |
| - | ===='''1 - | + | ===='''1 - Sequensis analysis of Bba_K1155000 ''==== |
| + | |||
| + | Abdou | ||
| + | |||
| + | WE OUR FIRST BIOBRICK ! | ||
| + | The sequence analysis was good. We call our biobrick Bba_K1155000. | ||
| + | |||
| + | ===='''Objective : obtaining Bba_K1155003, bba_K1155007'''==== | ||
| + | |||
| + | ===='''1 - Liquid culture of Bba_B0010, Bba_B0015, Bba_B0017, Bba_I732019 ''==== | ||
| + | |||
| + | Sheng, Zhou | ||
| + | |||
| + | {| | ||
| + | | style="border:1px solid black;padding:5px;background-color:#DE;" | | ||
| + | Tranformations of 07/12/13 works. We will do a liquid culture of them. | ||
| + | |} | ||
| + | |||
| + | We used 5 colonies of each plasmid. | ||
| + | |||
| + | ===='''2 - Digestion of Bba_B0010 by EcoRI/PstI ''==== | ||
| + | |||
| + | Anaïs | ||
| + | |||
| + | Used quantities : | ||
| + | * DNA : 20µL | ||
| + | * Buffer FD : 3µL | ||
| + | * EcoRI : 1µL | ||
| + | * PstI : 1µL | ||
| + | * H2O : 5µL | ||
| + | |||
| + | We let our digestion 1h30 at 37°C. | ||
| Line 37: | Line 68: | ||
We let the digestion 1h30 at 37°C. | We let the digestion 1h30 at 37°C. | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
===='''3 - PCR of digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3'''==== | ===='''3 - PCR of digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3'''==== | ||
| Line 50: | Line 74: | ||
Used quantities : | Used quantities : | ||
| + | ..... | ||
| + | |||
| + | COLONIES PIQUEES DANS 20µL d'eau pour chaque colonie. | ||
| + | |||
| + | Used quantities : | ||
| + | * BphA1 or BphR1 or BphR2 : 2µL | ||
| + | * Mix : (it was divided in 2 tubes for each oligo combinaison with 23µL of mix in each tube) | ||
| + | ** VF or Pfnr_Up : 6µL | ||
| + | ** VR or Pfnr_Down or VR : 6µL | ||
| + | ** dNTP : 6µL | ||
| + | ** Buffer Dream Taq : 30µL | ||
| + | ** Dream Taq : 6µL | ||
| + | ** H2O : 246µL | ||
| + | |||
.... | .... | ||
Revision as of 15:05, 25 September 2013
Contents |
Notebook : July 15
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155000
'1 - Sequensis analysis of Bba_K1155000
Abdou
WE OUR FIRST BIOBRICK ! The sequence analysis was good. We call our biobrick Bba_K1155000.
Objective : obtaining Bba_K1155003, bba_K1155007
'1 - Liquid culture of Bba_B0010, Bba_B0015, Bba_B0017, Bba_I732019
Sheng, Zhou
|
Tranformations of 07/12/13 works. We will do a liquid culture of them. |
We used 5 colonies of each plasmid.
'2 - Digestion of Bba_B0010 by EcoRI/PstI
Anaïs
Used quantities :
- DNA : 20µL
- Buffer FD : 3µL
- EcoRI : 1µL
- PstI : 1µL
- H2O : 5µL
We let our digestion 1h30 at 37°C.
B - PCB sensor system
Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2
1 - Digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments
Abdou
Used quantities :
- EcoRI/PstI :
- DNA : 8µL
- Buffer Orange : 1.6µL
- EcoRI : 1µL
- PstI : 1µL
- H2O : 4.4µL
- SacII :
- DNA : 8µL
- Buffer Blue : 1.6µL
- SacII : 1µL
- H2O : 5.4µL
We let the digestion 1h30 at 37°C.
3 - PCR of digestion of Bba_K11001, Bba_K1155002 and BphR2 in PSB1C3
Anaïs, Sheng, Zhou
Used quantities : .....
COLONIES PIQUEES DANS 20µL d'eau pour chaque colonie.
Used quantities :
- BphA1 or BphR1 or BphR2 : 2µL
- Mix : (it was divided in 2 tubes for each oligo combinaison with 23µL of mix in each tube)
- VF or Pfnr_Up : 6µL
- VR or Pfnr_Down or VR : 6µL
- dNTP : 6µL
- Buffer Dream Taq : 30µL
- Dream Taq : 6µL
- H2O : 246µL
....
PCR Program :
- BphR1 :
- BphA1, BphR2 :
| Previous week | Back to calendar | next day |
