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Team:Paris Saclay/Notebook/July/16
From 2013.igem.org
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Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]] | Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]] | ||
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| + | ===='''1 - Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI'''==== | ||
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| + | Anaïs | ||
| + | * DNA : 5µL | ||
| + | * Buffer FD : 2µL | ||
| + | * EcoRI FD : 1µL | ||
| + | * PstI FD : 1µL | ||
| + | * H2O : 11µL | ||
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| + | We let our digestion 1h30 at 37°C. | ||
===='''Objective : obtaining biobricks in PSB3K3'''==== | ===='''Objective : obtaining biobricks in PSB3K3'''==== | ||
Revision as of 16:37, 26 September 2013
Notebook : July 16
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003, Bba_K1155007
1 - Extraction of Bba_B0015, Bba_B0017, Bba_I732019 from DH5α
Zhou
Protocol : High copy plamid extraction
1 - Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI
Anaïs
- DNA : 5µL
- Buffer FD : 2µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- H2O : 11µL
We let our digestion 1h30 at 37°C.
Objective : obtaining biobricks in PSB3K3
1 - Digestion of PSB3K3 by EcoRI/PstI
Anaïs
Used quantities :
- DNA : 5µL
- Buffer FD : 2µL
- EcoRI FD : 1µL
- PstI FD : 1µL
- H2O : 11µL
We let our digestion 1h30 at 37°C.
2 - Electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
Sheng
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Expected sizes :
- PSB3K3 : 2750bp
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We obtain fragments at the right size. |
3 - Gel purification of electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
Abdou
Protocol : Gel purification
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