Team:Paris Saclay/Notebook/July/19

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Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
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Revision as of 19:33, 26 September 2013

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Contents

Notebook : July 19

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155003, Bba_K1155007

1 - Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017

Abdou, Anaïs

[[]]
  • Well 1 : 2µL Bba_B0015+1µL of 6X loading dye
  • Well 2 : 2µL Bba_B0017+1µL of 6X loading dye
  • Well 3 : 6µL DNA Ladder
  • Well 4 : 6µL DNA Ladder
  • Gel : 1%

Expected sizes :

  • Bba_B0015 digested by EcoRI/PstI : 500bp
  • Bba_B0017 digested by EcoRI/PstI : 500bp

We obtain fragments at the right size. We will digest them.

2 - Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017

Abdou, Anaïs

Protocol : Gel purification


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