Team:Paris Saclay/Notebook/July/2

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{{Team:Paris_Saclay/incl_debut_generique}}
{{Team:Paris_Saclay/incl_debut_generique}}
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='''Notebook : July 1'''=
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='''Notebook : July 2'''=
=='''Lab work'''==
=='''Lab work'''==
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[[File:PS_NP.jpg|right|200px]]
 
==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining Bba_K1155000'''====
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[[File:PS_NP.jpg|right|250px]]
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===='''Objective : obtaining BBa_K1155000'''====
===='''1 - Ligation concentration control by Nanodrop'''====
===='''1 - Ligation concentration control by Nanodrop'''====
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Nanodrop :  
Nanodrop :  
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* Bba_K1155000 : 411.7ng/µL
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* BBa_K1155000 : 411.7ng/µL
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===='''1 - Tranformation of Bba_I732017, Bba_K592009 and Bba_K1155000 in DH5α'''====
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===='''2 - Tranformation of BBa_K1155000 in DH5α'''====
Abdou, Zhou
Abdou, Zhou
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Protocol : [[Team:Paris_Saclay/Protocols/Bacterial transformation|Bacterial transformation]]
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Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.
We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.
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 +
===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
 +
 +
===='''1 - Tranformation of BBa_I732017, BBa_K592009 in DH5α'''====
 +
 +
Abdou, Zhou
 +
 +
Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
 +
 +
We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.
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=='''Human practices'''==
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We made the second meeting about open source : [[Team:Paris_Saclay/opensourcereflexion|Open Source reflexion]]
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{| border="1" align="center"

Latest revision as of 10:06, 30 September 2013

Contents

Notebook : July 2

Lab work

A - Aerobic/Anaerobic regulation system

PS NP.jpg

Objective : obtaining BBa_K1155000

1 - Ligation concentration control by Nanodrop

Sheng

Nanodrop :

  • BBa_K1155000 : 411.7ng/µL

2 - Tranformation of BBa_K1155000 in DH5α

Abdou, Zhou

Protocol : Bacterial transformation

We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.

Objective : obtaining BBa_K1155003, BBa_K1155007

1 - Tranformation of BBa_I732017, BBa_K592009 in DH5α

Abdou, Zhou

Protocol : Bacterial transformation

We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.


Human practices

We made the second meeting about open source : Open Source reflexion


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