Team:Paris Saclay/Notebook/July/2
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{{Team:Paris_Saclay/incl_debut_generique}} | {{Team:Paris_Saclay/incl_debut_generique}} | ||
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='''Notebook : July 2'''= | ='''Notebook : July 2'''= | ||
=='''Lab work'''== | =='''Lab work'''== | ||
- | + | ||
+ | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
+ | |||
+ | [[File:PS_NP.jpg|right|250px]] | ||
+ | |||
+ | ===='''Objective : obtaining BBa_K1155000'''==== | ||
+ | |||
+ | ===='''1 - Ligation concentration control by Nanodrop'''==== | ||
+ | |||
+ | Sheng | ||
+ | |||
+ | Nanodrop : | ||
+ | * BBa_K1155000 : 411.7ng/µL | ||
+ | |||
+ | ===='''2 - Tranformation of BBa_K1155000 in DH5α'''==== | ||
+ | |||
+ | Abdou, Zhou | ||
+ | |||
+ | Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] | ||
+ | |||
+ | We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations. | ||
+ | |||
+ | ===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''==== | ||
+ | |||
+ | ===='''1 - Tranformation of BBa_I732017, BBa_K592009 in DH5α'''==== | ||
+ | |||
+ | Abdou, Zhou | ||
+ | |||
+ | Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] | ||
+ | |||
+ | We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations. | ||
+ | |||
+ | |||
+ | =='''Human practices'''== | ||
+ | |||
+ | We made the second meeting about open source : [[Team:Paris_Saclay/opensourcereflexion|Open Source reflexion]] | ||
+ | |||
+ | |||
+ | {| border="1" align="center" | ||
+ | |[[Team:Paris Saclay/Notebook/July/1|<big>Previous day</big>]] | ||
+ | |||
+ | |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]] | ||
+ | |||
+ | |[[Team:Paris Saclay/Notebook/July/3|<big>Next day</big>]] | ||
+ | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} |
Latest revision as of 10:06, 30 September 2013
Contents |
Notebook : July 2
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155000
1 - Ligation concentration control by Nanodrop
Sheng
Nanodrop :
- BBa_K1155000 : 411.7ng/µL
2 - Tranformation of BBa_K1155000 in DH5α
Abdou, Zhou
Protocol : Bacterial transformation
We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - Tranformation of BBa_I732017, BBa_K592009 in DH5α
Abdou, Zhou
Protocol : Bacterial transformation
We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.
Human practices
We made the second meeting about open source : Open Source reflexion
Previous day | Back to calendar | Next day |