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Team:Paris Saclay/Notebook/July/2
From 2013.igem.org
(Difference between revisions)
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[[File:PS_NP.jpg|right|250px]] | [[File:PS_NP.jpg|right|250px]] | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155000'''==== |
===='''1 - Ligation concentration control by Nanodrop'''==== | ===='''1 - Ligation concentration control by Nanodrop'''==== | ||
| Line 16: | Line 16: | ||
Nanodrop : | Nanodrop : | ||
| - | * | + | * BBa_K1155000 : 411.7ng/µL |
| - | ===='''2 - Tranformation of | + | ===='''2 - Tranformation of BBa_K1155000 in DH5α'''==== |
Abdou, Zhou | Abdou, Zhou | ||
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We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations. | We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations. | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''==== |
| - | ===='''1 - Tranformation of | + | ===='''1 - Tranformation of BBa_I732017, BBa_K592009 in DH5α'''==== |
Abdou, Zhou | Abdou, Zhou | ||
Latest revision as of 10:06, 30 September 2013
Contents |
Notebook : July 2
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155000
1 - Ligation concentration control by Nanodrop
Sheng
Nanodrop :
- BBa_K1155000 : 411.7ng/µL
2 - Tranformation of BBa_K1155000 in DH5α
Abdou, Zhou
Protocol : Bacterial transformation
We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - Tranformation of BBa_I732017, BBa_K592009 in DH5α
Abdou, Zhou
Protocol : Bacterial transformation
We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.
Human practices
We made the second meeting about open source : Open Source reflexion
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