Notebook : July 2

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155000

1 - Ligation concentration control by Nanodrop

Sheng

Nanodrop :

• Bba_K1155000 : 411.7ng/µL

1 - Tranformation of Bba_I732017, Bba_K592009 and Bba_K1155000 in DH5α

Abdou, Zhou

Protocol : Bacterial transformation

We also made liquid culture with chloramphenicol or ampicilin at 37°C of our tranformations.