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Team:Paris Saclay/Notebook/July/23
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Xavier | Xavier | ||
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===='''5 - Electrophoresis to check the gel purification of digestion products : RBS-LacZ'''==== | ===='''5 - Electrophoresis to check the gel purification of digestion products : RBS-LacZ'''==== | ||
Revision as of 18:35, 25 September 2013
Notebook : July 23
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003, Bba_K1155007
1 - Electrophoresis of the digestion of Bba_I732017 by EcoRI/SpeI, Bba_K592009 SpeI/PstI, Bba_B0015, Bba_B0017 by XbaI/PstI
Abdou, Anaïs, Xavier, Zhou
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Expected sizes :
- RBS-LacZ :
- Amil CP :
- Term :
- PSB3A1 :
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We obtain a RBS-lacZ and Term fragments at the right size. The digestions were good and we will purify them. |
4 - Gel purification of digestion products : RBS-LacZ
Xavier
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
5 - Electrophoresis to check the gel purification of digestion products : RBS-LacZ
Abdou
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Expected sizes :
- RBS-LacZ :
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We can't see anything. The purification wasn't good. We will do the digestion again. |
6 - Digestion of Bba_I732017 by EcoRI/PstI
Anaïs
Used quantities :
- Bba_I732017 : 10µL
- Buffer FD : 3µL
- EcoRI FD : 1.5µL
- PstI FD : 1.5µL
- H2O : 14µL
7 - Electrophoresis to check the gel digestion of Bba_I732017 by EcoRI/SpeI
Zhou
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We can't see anything. The digestion wasn't good, we will do it the again. |
8 - Culture of Bba_I732017 and Bba_K592009
Abdou, Xavier
