Team:Paris Saclay/Notebook/July/30
From 2013.igem.org
Contents |
Notebook : July 30
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006
1 - Denaturation of EcoRI/SpeI used of the digestion of PCR products : NarK, NarG, NirB
Xavier
Protocol : Ethanol precipitation
We used 30µL of DNA.
Objective : obtaining Bba_K1155003
1 - Tranformation of RBS-AmilCP in PSB1C3 in DH5α
Abdou
Protocol : Bacterial transformation
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins (Gibson assembly)
1 - PCR of : BphR2 Part I, BphR2 Part II, RBS-BphR2 Part I, FNR Part I, FNR Part II, RBS-FNR Part I
Abdou, Xavier
Used quantities :
- Bphr2 Part I :
- Oligo 54F : 4 µL
- Oligo 55R :4 µL
- Buffer .... : 40 µL
- DNA : 2 µL
- dNTP : 4 µL
- .... : 1 µL
- H2O : 146 µL
- Bphr2 Part II :
- Oligo 54F : 4 µL
- Oligo 55R :4 µL
- Buffer .... : 40 µL
- DNA : 2 µL
- dNTP : 4 µL
- .... : 1 µL
- H2O : 146 µL
- RBS-Bphr2 Part I :
- Oligo 54F : 4 µL
- Oligo 55R :4 µL
- Buffer .... : 40 µL
- DNA : 2 µL
- dNTP : 4 µL
- .... : 1 µL
- H2O : 146 µL
- FNR Part I :
- Oligo ... : 4 µL
- Oligo ... :4 µL
- Buffer .... : 40 µL
- DNA : 2 µL
- dNTP : 4 µL
- .... : 1 µL
- H2O : 146 µL
- FNR Part II :
- Oligo ... : 4 µL
- Oligo ... :4 µL
- Buffer .... : 40 µL
- DNA : 2 µL
- dNTP : 4 µL
- .... : 1 µL
- H2O : 146 µL
- RBS-FNR Part I :
- Oligo ... : 4 µL
- Oligo ... :4 µL
- Buffer .... : 40 µL
- DNA : 2 µL
- dNTP : 4 µL
- .... : 1 µL
- H2O : 146 µL
- PSB1C3 :
- Prefixe ... : 4 µL
- Suffixe ... :4 µL
- Buffer .... : 40 µL
- DNA : 2 µL
- dNTP : 4 µL
- .... : 1 µL
- H2O : 146 µL
PCR Program :
- BphR2 Part I, BphR2 Part II, RBS-BphR2 Part I :
[File:PsPCRBphR23007.jpg|400px]]