Team:TU-Delft

From 2013.igem.org

(Difference between revisions)
 
(174 intermediate revisions not shown)
Line 3: Line 3:
{{:Team:TU-Delft/Templates/Frog}}
{{:Team:TU-Delft/Templates/Frog}}
{{:Team:TU-Delft/Templates/Logo}}
{{:Team:TU-Delft/Templates/Logo}}
 +
<html>
<html>
 +
<div style="margin-top:-20px;margin-bottom:5px;">
 +
<center>
 +
<a href="https://static.igem.org/mediawiki/2013/8/82/Pep9.jpg">
 +
<img src="https://static.igem.org/mediawiki/2013/8/82/Pep9.jpg" alt=""  HEIGHT=90" WIDTH="700" />
 +
</a>
 +
</center>
 +
</div>
 +
 +
<!--copyright METU TEAM iGem 2012-->
<head>
<head>
<meta http-equiv="Content-type" content="text/html; charset=utf-8" />
<meta http-equiv="Content-type" content="text/html; charset=utf-8" />
Line 21: Line 31:
<style>
<style>
body{
body{
 +
background:url("https://static.igem.org/mediawiki/2013/3/32/BackroundDelft.jpg");
 +
background-repeat:no-repeat;
border-top: none;
border-top: none;
Line 34: Line 46:
}
}
.slider-right{
.slider-right{
-
width:637px;
+
width:610px;
}
}
Line 57: Line 69:
<ul>
<ul>
    <li>
    <li>
-
    <h2>Who are we?</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">We are iGEM 2013 team of TU Delft, Netherlands. We are working on the production of antimicrobial peptides in order to kill multiple resistant <i> Staphyllococcus aureus</i>.</p>
+
    <h2>Who are we?</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">We are the iGEM 2013 team of Delft University of Technology, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant <i>Staphylococcus aureus, MRSA</i>.</p>
    </li>
    </li>
 +
 +
<li> <h2>Aim</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> To engineer <i>Escherichia coli</i> that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules.
 +
</p>
 +
 +
                                     
 +
    </li>
 +
    <li>
    <li>
-
    <h2>Sensing AIP</h2>   
+
    <h2>Sensing autoinducing peptides</h2>   
-
    <div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">The sensing part of the Agr system was engineered in E.coli. In that way E.coli detects the AIPs that are produced by the Agr system of S.aureus and the peptide is produced. </p>
+
    <div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">The sensing part of the of <i>Staphylococcus aureus</i> Agr system is engineered in <i>E. coli</i>. In that way <i>E. coli</i> detects the AIPs that are produced by <i>Staphylococcus aureus</i> which  induces the peptide production. </p>
                                        
                                        
Line 68: Line 87:
    <li>
    <li>
    <h2>Peptide Production</h2>
    <h2>Peptide Production</h2>
-
    <h8>######</h8>
+
<div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> <i>E. coli</i> detects <i>Staphylococcus aureus</i> by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are  being produced </p>
    </li>
    </li>
      <li>
      <li>
    <h2>Timer</h2>
    <h2>Timer</h2>
-
    <h8>######.</h8>
+
<div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">
 +
With the use of a timer the peptide will be activated by cleavage of an inactivating tag. With the use of a timer, high concentrations of peptide can be delivered very locally in order to efficiently kill MRSA.</p>
 +
 
    </li>
    </li>
                                   <li>
                                   <li>
    <h2>Kill Switch</h2>
    <h2>Kill Switch</h2>
-
    <h8>######.</h8>
+
<div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">
 +
In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.</p>  
    </li>
    </li>
-
       
+
   
 +
<li>
 +
    <h2>Zephyr</h2>
 +
<div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">
 +
Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale.
 +
</p>
 +
    </li>
 +
   
 +
 
 +
   
</ul>
</ul>
</div>
</div>
Line 85: Line 116:
    <li>
    <li>
<a href="https://2013.igem.org/Team:TU-Delft/Team">
<a href="https://2013.igem.org/Team:TU-Delft/Team">
-
<img src="https://static.igem.org/mediawiki/2013/b/b3/TeamDelft.jpg" alt="" HEIGHT="348" WIDTH="600" />
+
<img src="https://static.igem.org/mediawiki/2013/7/7f/Team_Delft1.jpg" alt="" HEIGHT="348" WIDTH="600" />
</a>
</a>
                                     </li>
                                     </li>
 +
 +
<li>
 +
<a href="https://2013.igem.org/Team:TU-Delft/ProjectOverview">
 +
<img src="https://static.igem.org/mediawiki/2013/5/51/Schem-new.jpg" alt="" HEIGHT="348" WIDTH="600"/>
 +
</a>
 +
 +
</li>
 +
 +
    <li>
    <li>
-
<a href="#">
+
<a href="https://2013.igem.org/Team:TU-Delft/Sensing">
-
<img src="https://static.igem.org/mediawiki/2013/6/6a/AIP.jpg" alt="" HEIGHT="348" WIDTH="600"/>
+
<img src="https://static.igem.org/mediawiki/2013/6/6d/Sensing-AIP.jpg" alt="" HEIGHT="348" WIDTH="600"/>
</a>
</a>
                                     </li>
                                     </li>
    <li>
    <li>
-
<a href="#">
+
<a href="https://2013.igem.org/Team:TU-Delft/Peptides">
-
<img src="#" alt="" HEIGHT="348" WIDTH="660"/>
+
<img src="https://static.igem.org/mediawiki/2013/f/fe/Peptide-2.jpg" alt="" HEIGHT="348" WIDTH="600"/>
</a>
</a>
</li>
</li>
    <li>
    <li>
-
<a href="#">
+
<a href="https://2013.igem.org/Team:TU-Delft/Timer">
-
<img src="#" alt="" HEIGHT="348" WIDTH="660"/>
+
<img src="https://static.igem.org/mediawiki/2013/3/34/Timer-2.jpg" alt="" HEIGHT="348" WIDTH="600"/>
</a>
</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2013.igem.org/Team:TU-Delft/Killswitch">
 +
<img src="https://static.igem.org/mediawiki/2013/f/f8/Kill2.jpg" alt="" HEIGHT="348" WIDTH="600"/>
 +
</a></li>
 +
 +
<li>
 +
<a href="https://2013.igem.org/Team:TU-Delft/Zephyr">
 +
<img src="https://static.igem.org/mediawiki/2013/4/4c/2D_table_ZephyrHome.jpg" alt="" WIDTH="600"/>
 +
</a>
 +
</li>
</li>
Line 108: Line 160:
<div class="cl">&nbsp;</div>
<div class="cl">&nbsp;</div>
<div class="slider-nav">
<div class="slider-nav">
-
<a href="#">1</a>
+
<a href="https://2013.igem.org/Team:TU-Delft/Team">1</a>
-
<a href="#">2</a>
+
                              <a href="https://2013.igem.org/Team:TU-Delft/ProjectOverview">2</a>
-
<a href="#">3</a>
+
<a href="https://2013.igem.org/Team:TU-Delft/Sensing">3</a>
-
<a href="#">4</a>
+
<a href="https://2013.igem.org/Team:TU-Delft/Peptides">4</a>
-
                                <a href="#">5</a>
+
<a href="https://2013.igem.org/Team:TU-Delft/Timer">5</a>
 +
                                <a href="https://2013.igem.org/Team:TU-Delft/Killswitch">6</a>
 +
<a href="https://2013.igem.org/Team:TU-Delft/Zephyr">7</a>
<div class="cl">&nbsp;</div>
<div class="cl">&nbsp;</div>
Line 123: Line 177:
<br>
<br>
-
<div style="margin-left:0px;width:200px;float:left;display:inline-block;">   
+
<div style="margin-left:40px;width:420px;float:left;display:inline-block;">   
-
<h2>Sponsors</h2>
+
<br>
-
<img src="https://static.igem.org/mediawiki/2013/1/17/AMPLINO-TUDelft.jpg" alt="" HEIGHT="100" WIDTH="200" />
+
 
-
</div>
+
-
<div style="margin-left:150px;width:100px;float:left;display:inline-block;"> 
 
-
<br>
 
<script>(function(d, s, id) {
<script>(function(d, s, id) {
   var js, fjs = d.getElementsByTagName(s)[0];
   var js, fjs = d.getElementsByTagName(s)[0];
Line 146: Line 197:
<img src="http://www.easycounter.com/counter.php?tudelft3"
<img src="http://www.easycounter.com/counter.php?tudelft3"
border="0" alt="Free Web Counters"></a>
border="0" alt="Free Web Counters"></a>
 +
</div>
 +
 +
<div style="margin-left:50px;width:200px;display:inline-block;"> 
 +
<object width="420" height="315"><param name="movie" value="//www.youtube.com/v/jMUHYKS2jvc?hl=el_GR&amp;version=3"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/jMUHYKS2jvc?hl=el_GR&amp;version=3" type="application/x-shockwave-flash" width="420" height="315" allowscriptaccess="always" allowfullscreen="true"></embed></object>
 +
</div>
<br>
<br>
 +
<br>
 +
 +
<center>
 +
<p> <h3> Check out what news in Netherlands has to say about the achievements of our Team </p><a href="https://2013.igem.org/Team:TU-Delft/PR" target="blank"> In News. </a> </h3> <br> </center>
 +
 +
<div style="margin-top:10px;margin-left:400px;float:left;display:inline-block;"> 
 +
<h2>Our sponsors</h2>
 +
<hr>
 +
</div>
 +
<br>
 +
<br>
 +
<br>
 +
<center>
 +
<table style="border-spacing: 3px; alignment: center;" cellspacing="0" cellpadding="0">
 +
<tr >
 +
<td colspan="4" style="text-align: center;"><h3></h3></td>
 +
</tr>
 +
 +
<tr>
 +
<!--<td style="text-align: center;" width="250px">
 +
<a href="http://www.bt.tudelft.nl/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/thumb/e/e6/TUD_BT.png/180px-TUD_BT.png"  width=177 height=60></a>
 +
 +
</td>-->
 +
 +
<!--BT--> <td style="text-align: center;" width="250px">
 +
<a href="http://home.tudelft.nl/en" target="_blank"><img src="https://static.igem.org/mediawiki/igem.org/5/5a/Logotudelft.png" width=215 height=60></a>
 +
 +
</td>
 +
<!--BN--> <td style="text-align: center;" width="250px">
 +
 +
<a href="http://www.bt.tudelft.nl/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/thumb/e/e6/TUD_BT.png/180px-TUD_BT.png"  width=177 height=60></a>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td style="text-align: center;" width="250px">
 +
<!--dcsc-->
 +
<a href="http://www.tnw.tudelft.nl" target="_blank"><img src="https://static.igem.org/mediawiki/2012/thumb/0/02/TUD_BioNanoScience.jpg/180px-TUD_BioNanoScience.jpg" width=276 height=60></a>
 +
</td>
 +
<td style="text-align: center;" width="250px">
 +
<!--Bio--> <a href="http://bioinformatics.tudelft.nl/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/thumb/4/4e/TUD_BioInformatics.png/180px-TUD_BioInformatics.png" width=144 height=60> </a>
 +
</td>
 +
</tr>
-
<iframe width="420" height="345"
+
<tr>
-
src="http://www.youtube.com/embed/XGSy3_Czz8k">
+
<td style="text-align: center;" width="250px">
-
</iframe>
+
<a href="http://www.be-basic.org/fileadmin/custom/images/logo-new.png" target="_blank"><img src="https://static.igem.org/mediawiki/2013/9/9b/Bebasic.png" width=88 height=60> </a>
 +
</td>
 +
<td style="text-align: center;" width="250px">
 +
<a href="http://stud.nl/" target="_blank"><img src="https://static.igem.org/mediawiki/2012/thumb/7/75/TUD_stuD.jpg/180px-TUD_stuD.jpg" width=136 height=60> </a>
 +
</td>
 +
<td style="text-align: center;">
 +
</td>
 +
</tr>
 +
<tr>
 +
<td style="text-align: center;" width="250px">
 +
<a href="https://static.igem.org/mediawiki/2013/archive/5/58/20131028223200!Logo_ERASynBio.png"target="_blank"><img src=" https://static.igem.org/mediawiki/2013/archive/5/58/20131028223200!Logo_ERASynBio.png"width=120 height=40> </a>
 +
</td>
 +
</tr>
 +
</table>
 +
</center>
</div>
</div>

Latest revision as of 22:37, 28 October 2013


  • Who are we?

    We are the iGEM 2013 team of Delft University of Technology, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant Staphylococcus aureus, MRSA.

  • Aim

    To engineer Escherichia coli that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules.

  • Sensing autoinducing peptides

    The sensing part of the of Staphylococcus aureus Agr system is engineered in E. coli. In that way E. coli detects the AIPs that are produced by Staphylococcus aureus which induces the peptide production.

  • Peptide Production

    E. coli detects Staphylococcus aureus by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are being produced

  • Timer

    With the use of a timer the peptide will be activated by cleavage of an inactivating tag. With the use of a timer, high concentrations of peptide can be delivered very locally in order to efficiently kill MRSA.

  • Kill Switch

    In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.

  • Zephyr

    Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale.

 
1 2 3 4 5 6 7
 



Locations of visitors to this page Free Web Counters


Check out what news in Netherlands has to say about the achievements of our Team

In News.


Our sponsors

Retrieved from "http://2013.igem.org/Team:TU-Delft"