Team:TU-Delft/FinalApplication

BandAid Application

Experiments

Aim: To check if it is feasible for the peptides to pass through the selected membranes (Cellulose Nitrate Membrane and PTFE Membrane).

1. Dissolve the peptides. Signiferin was dissolved in water, in order to dissolve maximim 1M of NH4HCO3 was added in order to gain an end concentration of 5mM.
2. The peptide solutions were measured, using the nanodrop, and we determined to have an absorbance of 0.74 and 0.51 respectively.Using absorbance A= εcL=εc when L = 1cm [1] the concentration was measured to be 0.49mM for signiferin and 0.34mM for maximim
3. In order to make diffusion measurable, we folded the membrane like a filter paper and placed it in the peptide solution
5. The membrane is add




	Absorbance(Maximim)	Absorbance(Signiferin)	Concentration in mM Maximim	Concentration in mM Signiferin
∅	2.83	1.76	1.89	1.18
t0	0	0	0	0
t10	0.4	0.64	0.26	0.42
t20	0.93	0.71	0.62	0.47
t30	1.98	1.46	1.32	0.97

Table 1: Cellulose Nitrate Membrane, 0.2μm pore size

	Absorbance(Maximim)	Absorbance(Signiferin)	Concentration in mM Maximim	Concentration in mM Signiferin
∅	2.83	1.76	1.89	1.18
t0	0	0	0	0
t10	0	0	0	0
t20	0	0	0	0
t30	0	0	0	0

Table 2: PTFE Membrane, 0.2μm pore size

	Absorbance(Maximim)	Absorbance(Signiferin)	Concentration in mM Maximim	Concentration in mM Signiferin
∅	0.51	0.74	0.02	0.49
t1	0	0	0	0
t2	0.03	0	0.02	0
t3	0.01	0.08	0.006	0.05
t20	0.02	_	0.013	-
t60	0.16	0.27	0.1	0.18
t80	0.2	0.44	0.13	0.29

Table 3: Cellulose Nitrate Membrane, 0.1μm pore size

	Absorbance(Maximim)	Absorbance(Signiferin)	Concentration in mM Maximim	Concentration in mM Signiferin
∅	0.51	0.74	0.02	0.49
t1	0	0	0	0
t2	0	0	0	0
t3	0	0	0	0
t20	0	0	0	0
t60	0	0	0	0
t80	0	0	0	0

Table 4: PTFE Membrane, 0.1μm pore size