Team:TU-Eindhoven/Results

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These experiments resulted in the observation that we have two more functional Biobricks: [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1123000 BBa_K1123001] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1123005 BBa_K1123005].  
These experiments resulted in the observation that we have two more functional Biobricks: [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1123000 BBa_K1123001] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1123005 BBa_K1123005].  
To see detailed information and results, see [https://2013.igem.org/Team:TU-Eindhoven/AnearobicTesting Anaerobic FNR Testing].
To see detailed information and results, see [https://2013.igem.org/Team:TU-Eindhoven/AnearobicTesting Anaerobic FNR Testing].
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=== Overall Result ===

Revision as of 16:36, 4 October 2013

Contents

Aerobic Expression

In the experiments for the aerobic expression of the proteins, we have seen that we have produced functional Biobricks. The proteins 1ETF, 1PJN, 1G70 and EGFP were produced aerobically so that we can say that the following Biobricks were succesfull: [http://parts.igem.org/Part:BBa_K1123015 BBa_K11230014], [http://parts.igem.org/Part:BBa_K1123015 BBa_K1123015], [http://parts.igem.org/Part:BBa_K1123016 BBa_K1123016] and [http://parts.igem.org/Part:BBa_K1123015 BBa_K1123017]. For more detailed information and further results obtained by these experiments, see CEST Agent Testing.

CEST Agent Testing

Anaerobic FNR Testing

After analyzing the samples taken during anaerobically induced expression of EGFP, we can conclude that the FNR promoter is a functional Biobrick. We can conclude that the EGFP Protein Production Construct is functional too since EGFP was produced.

TU-Eindhoven Images SDSgelXL1blue.jpg
SDSgelXL1blue 12% SDS gel with the bugbustered samples of the XL1-Blue anaerobic expressions of EGFP.
TU-Eindhoven Images SDSgelXL1bluepur.jpg
{{{id}}} 12% SDS gel with the purified samples of the XL1-Blue anaerobic expressions of EGFP

We performed Fluorescence measurments of the samples taken at different time points during the anaerobically induced expression of EGFP.

TU-Eindhoven Images Fluorescencemeasurements.jpg
Fluorescencemeasurements Emission spectra of EGFP from the XL1-Blue anaerobic expression samples
TU-Eindhoven Images Emissionpeaks.jpg
Emissionpeaks Values of the peaks of the emission spectra of EGFP per sample
These experiments resulted in the observation that we have two more functional Biobricks: [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1123000 BBa_K1123001] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1123005 BBa_K1123005]. To see detailed information and results, see Anaerobic FNR Testing.

Overall Result