Team:TU-Munich/Results/GM-Moss

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Transformation of Physcomitrella patens












Figure 1: Work flow for the generation of transgenic moss.


1 Generation of expression constructs

Table 1: Transgenic Physcomitrella patnes plants
Number Construct name (abbreviation) BioBrick Figure Size [bp] Lane
PF-1 GFP (GFP-cyt) <partinfo>BBa_E0040</partinfo> x
PF-2 Igk-GFP (GFP-sec) <partinfo>BBa_E0040</partinfo> x
PF-3 NanoLuciferase (nLuc-cyt) <partinfo>BBa_E0040</partinfo> x
PF-4 Igk-NanoLuciferase (nLuc-sec) <partinfo>BBa_E0040</partinfo> x
PF-5 SERK-NanoLuciferase (SERK-nLuc) x
PF-6 NanoLuciferase-Receptor (nLuc-rec) <partinfo>BBa_E0040</partinfo> x
PF-7 Erythromycinesterase (EreB-cyt) <partinfo>BBa_E0040</partinfo> x
PF-8 Erythromycinesterase-Receptor (EreB-rec) <partinfo>BBa_E0040</partinfo> #
PF-9 Ig Kappa Erythromycinesterase (EreB-sec) <partinfo>BBa_E0040</partinfo> #
PF-10 Laccase-Receptor (Lac-rec) #
PF-11 Ig Kappa Laccase (Lac-sec) #
PF-12 Catecholdioxygenase (XylE-cyt) <partinfo>BBa_E0040</partinfo> x
PF-13 DDT-dehydrochlorinase (GST-cyt) <partinfo>BBa_E0040</partinfo> #
PF-14 PP1-Receptor (PP1-rec) <partinfo>BBa_E0040</partinfo> x
PF-15 FluA-Receptor (FluA-rec) <partinfo>BBa_E0040</partinfo> x
PF-16 Stressinducible_Promoter-RFP (Stress) <partinfo>BBa_E0040</partinfo> #
PF-17 SpyCatcher-Receptor:Spytag-nLuc (Catcher:Tag-nLuc) <partinfo>BBa_E0040</partinfo> #
PF-18 SpyCatcher-Receptor:nLuc-Spytag (Catcher:nLuc-Tag <partinfo>BBa_E0040</partinfo> #
PF-19 SpyTag-Receptor:SpyCatcher-nLuc (Tag:Catcher-nLuc) <partinfo>BBa_E0040</partinfo> #
PF-20 SpyTag-Receptor:nLuc-SpyCatcher (Tag:nLuc-Catcher) <partinfo>BBa_E0040</partinfo> #
PF-21 Alcohol acetyltransferase I (Banana) <partinfo>BBa_J45014</partinfo> #
PF-22 Kill-switch with PIF3 (PIF-3) <partinfo>BBa_E0040</partinfo> x
PF-23 Kill-switch with PIF6 (PIF-6) <partinfo>BBa_E0040</partinfo> x
PF-24 Kill-switch with PIF6 (FRET) <partinfo>BBa_E0040</partinfo> #
PF-25 Kill-switch with PIF3 (FRET) <partinfo>BBa_E0040</partinfo> #


2 Preparation of linear DNA

Figure 1: Work flow for the generation of transgenic moss.


3 Transformation of Physcomitrella patens

4 Regeneration and Selektion of transgenic plants

5 Characterization of transgenic plants

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.




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