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Team:Tokyo-NoKoGen/ikalux
From 2013.igem.org
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| + | <p align=center><font size=6> Steps in preparing ika culture</p></font> | ||
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<font size=4>As shown in the video, the steps needed for making a squid is very simple. The photos below will help you remember how the steps were taken. First you buy a fresh ika, then take the inside away and cut it into an appropriate size. Put that piece into a container filled with 3% NaCl. Leave this ika for 1 to 2 weeks at 4℃, you will soon start to see luminescent "spots" on the surface of ika - luminescent bacteria!An agarose gel can be easily made from boiling the rest of ika for 3 to 5 minutes, and mix it well with agar.</font> | <font size=4>As shown in the video, the steps needed for making a squid is very simple. The photos below will help you remember how the steps were taken. First you buy a fresh ika, then take the inside away and cut it into an appropriate size. Put that piece into a container filled with 3% NaCl. Leave this ika for 1 to 2 weeks at 4℃, you will soon start to see luminescent "spots" on the surface of ika - luminescent bacteria!An agarose gel can be easily made from boiling the rest of ika for 3 to 5 minutes, and mix it well with agar.</font> | ||
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<font size=4>ビデオでもお見せした通り、イカを用意するのはとても簡単です。下の写真は、ビデオでお見せした内容の復習です。まず、近所のスーパーで新鮮なイカを買ってきます。そのイカをちょうど良い大きさに切りだします。切り出した断片を3% NaClに浸し、４℃で１週間から２週間程度培養します。すると、イカの表面に光る”斑点”が見えてきますが、これが発光バクテリアです！寒天培地もとても簡単に用意できます。余ったイカをお鍋で３分から5分にて、寒天と混ぜるだけです。 | <font size=4>ビデオでもお見せした通り、イカを用意するのはとても簡単です。下の写真は、ビデオでお見せした内容の復習です。まず、近所のスーパーで新鮮なイカを買ってきます。そのイカをちょうど良い大きさに切りだします。切り出した断片を3% NaClに浸し、４℃で１週間から２週間程度培養します。すると、イカの表面に光る”斑点”が見えてきますが、これが発光バクテリアです！寒天培地もとても簡単に用意できます。余ったイカをお鍋で３分から5分にて、寒天と混ぜるだけです。 | ||
| - | <img src=https://static.igem.org/mediawiki/2013/5/5a/I01.jpg width=300> | + | <BR><img src=https://static.igem.org/mediawiki/2013/5/5a/I01.jpg width=300> |
<img src=https://static.igem.org/mediawiki/2013/d/de/I02.jpg width=300> | <img src=https://static.igem.org/mediawiki/2013/d/de/I02.jpg width=300> | ||
<img src=https://static.igem.org/mediawiki/2013/b/b7/I03.jpg width=300> | <img src=https://static.igem.org/mediawiki/2013/b/b7/I03.jpg width=300> | ||
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| + | <p align=center><font size=6> Obtain the luminescent bacteria</p></font> | ||
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| + | <BR>We were not satisfied with only finding the luminescent bacteria, but we also wanted to know what it is. Therefore we decided to culture the colony on the agar plate to get a single colony. Can you see it? | ||
| + | <BR>私達は発光バクテリアを見つけるだけでは満足が出来ませんでした。そこで、バクテリアの正体を探るために、寒天培地でバクテリアを培養してみました。見えるかな？ | ||
<img src=https://static.igem.org/mediawiki/2013/8/86/Lumi.jpg> | <img src=https://static.igem.org/mediawiki/2013/8/86/Lumi.jpg> | ||
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Revision as of 23:36, 27 September 2013
Human practice
- What is synthetic biology?
- How to work safely in the laboratory
- Questionnaire on Synthetic Biology
- Do It Yourself -IkaLux-
- Team interactions: iGEM JAPAN
DO IT YOURSELF
Simple Experiment to do at home
Please have a look at our video first
We hope you enjoyed our video! The video shows you how it is easy to culture a luminescent bacteria at home, a very simple experiment with only a need to buy a fresh squid from a nearby supermarket. The boy and a girl shown in the video are 1st year students at our university, who have no experience in doing laboratory experiments. Even they can do it for the first time - why don't you also try?
ビデオは楽しんでいただけたでしょうか？このビデオでは、光るバクテリアを家で簡単に培養出来るということ、スーパーで新鮮なイカを買ってくるだけで出来てしまうということを伝えたく、作りました。ビデオに登場する男の子と女の子は大学１年生で、まだ複雑な実験を経験したことがありません。あなたもぜひ家で培養してみませんか？
Steps in preparing ika culture
As shown in the video, the steps needed for making a squid is very simple. The photos below will help you remember how the steps were taken. First you buy a fresh ika, then take the inside away and cut it into an appropriate size. Put that piece into a container filled with 3% NaCl. Leave this ika for 1 to 2 weeks at 4℃, you will soon start to see luminescent "spots" on the surface of ika - luminescent bacteria!An agarose gel can be easily made from boiling the rest of ika for 3 to 5 minutes, and mix it well with agar.
ビデオでもお見せした通り、イカを用意するのはとても簡単です。下の写真は、ビデオでお見せした内容の復習です。まず、近所のスーパーで新鮮なイカを買ってきます。そのイカをちょうど良い大きさに切りだします。切り出した断片を3% NaClに浸し、４℃で１週間から２週間程度培養します。すると、イカの表面に光る”斑点”が見えてきますが、これが発光バクテリアです！寒天培地もとても簡単に用意できます。余ったイカをお鍋で３分から5分にて、寒天と混ぜるだけです。
Obtain the luminescent bacteria
We were not satisfied with only finding the luminescent bacteria, but we also wanted to know what it is. Therefore we decided to culture the colony on the agar plate to get a single colony. Can you see it?
私達は発光バクテリアを見つけるだけでは満足が出来ませんでした。そこで、バクテリアの正体を探るために、寒天培地でバクテリアを培養してみました。見えるかな？
