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Team:UANL Mty-Mexico/beta/1
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| - | <p> | + | <p>We developed a model for the characterization of the dynamic behavior of RNA thermometers. We propose some of the parameters of our model to be used as quantitative points of reference to help us understand and reliably compare the behavior of RNA thermometers.</p> |
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| - | <h3> | + | <h3>Human practices</h3> |
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Revision as of 02:41, 5 September 2013
UANL México
iGEM UANL MX
Our wiki is still under construction. It will be up and running very soon, please come back later. Thanks for visiting!
Project
We intend to build a temperature-regulated genetic circuit with three different states. The transition among these states is regulated by RNA thermometers: one whose optimal temperature is 32°C and the other is 37°C. These RNA thermometers will regulate directly the transcription of two different reporter proteins (GFP and RFP) and a transcription factor (LacI). Together with a thermolabile variant of transcription factor cI, these elements are integrated so that three different states emerge in dependence to temperature changes.
Our main purpose is to provide a proof of concept for the use of RNA thermometers to regulate the expression of a transcription factor in artificial genetic circuits. We also provide guidelines for the standard characterization of other RNA thermometers and point at possible applications.
Math
We developed a model for the characterization of the dynamic behavior of RNA thermometers. We propose some of the parameters of our model to be used as quantitative points of reference to help us understand and reliably compare the behavior of RNA thermometers.
Human practices
Donec sed odio dui. Cras justo odio, dapibus ac facilisis in, egestas eget quam. Vestibulum id ligula porta felis euismod semper. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa.
