Team:UC Davis

From 2013.igem.org

(Difference between revisions)
 
(185 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:UC_Davis/Head}}
 +
<html>
<html>
 +
 +
<head>
 +
     
 +
 +
 +
<!-- Start twitter javascript -->
 +
<script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)                                                                          [0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}  (document,"script","twitter-wjs");
 +
</script>
 +
 +
<script type="text/javascript" src="https://2013.igem.org/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 +
 +
<script>!function(d,s,id){var js,fjs=d.getElementsByTagName(s)[0],p=/^http:/.test(d.location)?'http':'https';if(!d.getElementById(id)){js=d.createElement(s);js.id=id;js.src=p+"://platform.twitter.com/widgets.js";fjs.parentNode.insertBefore(js,fjs);}}(document,"script","twitter-wjs");
 +
</script>
 +
 +
 +
<!-- end twitter javascript -->
 +
 +
 +
 +
<!-- Head Scripts -->
 +
<script src="https://2013.igem.org/wiki/skins/common/ajax.js?270"></script>
 +
<script src="https://2013.igem.org/wiki/skins/common/mwsuggest.js?270"></script>
 +
        <script type="text/javascript" src="https://2013.igem.org/wiki/index.php? title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 +
    <!-- jQuery Javascript -->
 +
    <script type="text/javascript"        src ="https://2013.igem.org/common/jquery-latest.min.js"></script>
 +
<!-- End head Scripts -->
 +
 +
 +
 +
 +
<style type="text/css">
 +
 +
img.checkmark{
 +
margin-right:2px;
 +
width:20px;
 +
height:16px;
 +
vertical-align:text-bottom;
 +
padding-right:1px;}
 +
 +
 +
.sponsorfloat {
 +
background-color: rgba(0,0,0,.65);
 +
margin-top: 10px;
 +
border-radius: 4px;
 +
padding: 5px 10px 12px 10px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: rgba(225,225,225,1);
 +
float:right
 +
}
 +
 +
#twitter {float:right; width:220px; height:300px; margin-top: 10px; opacity:0.8; rgba(0,0,0,.65);}
 +
 +
img.banner
 +
{
 +
opacity:0.8;
 +
filter:alpha(opacity=80); /* For IE8 and earlier */
 +
}
 +
 +
 +
 +
</style>
 +
 +
</head>
 +
 +
 +
<body>
<body>
-
<img src="https://static.igem.org/mediawiki/2013/d/d0/UC_Davis_picnic_day.jpg" />
 
-
<div id="overview"><h1>PROJECT OVERVIEW</h1>
 
-
<p>Transcription activator-like effectors (TALEs) are proteins secreted by the bacterial pathogen Xanthomonas that contain sequence specific DNA binding domains and can act as transcriptional repressors or activators (Mahfouz et al 2012).The DNA binding domains are sequence specific due to consecutive protein repeats, the composition of each which corresponds to a certain base preference (Meckler et al). TAL repressors can therefore be engineered to bind to any DNA sequence of interest, following now well-understood rules for TAL-DNA binding (Boch J et al 2009, Moscou et al 2009).  TALEs are thus a powerful and modular tool for the control of gene expression in genetic circuits. Current efforts to quantify and predict TALE binding affinities and functionalities are being made in order to create libraries of TALE systems that will serve to streamline research and the development of genetic devices (Meckler et al ).</p></div>
 
 +
<div><img src="https://static.igem.org/mediawiki/2013/1/19/UCD_2013_Banner_v2.PNG" class="banner" width=967px height=226/>
 +
</div>
-
</html>
+
<div class="sponsorsbox">
 +
        <div class="sponsorfloat">
 +
<h2>Our Sponsors</h2>
 +
<center>
 +
<a href="http://engineering.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2011/f/f6/UCD_CoE.png" width="200" height="40"></a>
 +
</center>
 +
<center>
 +
<a href="http://biosci.ucdavis.edu/index_js.html" target="_blank"><img src="https://static.igem.org/mediawiki/2011/b/b1/UCD_biosci_sponsor.jpg" width="200" height="90"></a>
 +
</center>
-
<!--- The Mission, Experiments --->
+
<center>
 +
<a href="http://www.genomecenter.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2011/1/1b/UCD_Genome_center_sponsor.jpg" width="200" height="60"></a>
 +
</center>
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<center>
-
!align="center"|[[Team:UC_Davis|Home]]
+
<a href="http://www.idtdna.com/site" target="_blank"><img src="https://static.igem.org/mediawiki/2012/2/22/IDT-Logo.jpg" width="200"></a>
-
!align="center"|[[Team:UC_Davis/Team|Team]]
+
</center>
-
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=UC_Davis Official Team Profile]
+
 
-
!align="center"|[[Team:UC_Davis/Project|Project]]
+
<center>
-
!align="center"|[[Team:UC_Davis/Parts|Parts Submitted to the Registry]]
+
<a href="http://www.bme.ucdavis.edu/" target="_blank"><img src="https://static.igem.org/mediawiki/2011/4/40/UCD_BME_logo_minimal_copy.png" width="200 height="70"></a>
-
!align="center"|[[Team:UC_Davis/Modeling|Modeling]]
+
</center>
-
!align="center"|[[Team:UC_Davis/Notebook|Notebook]]
+
 
-
!align="center"|[[Team:UC_Davis/Safety|Safety]]
+
</div>
-
!align="center"|[[Team:UC_Davis/Attributions|Attributions]]
+
</div>
-
|}
+
 
 +
<!--Begin Showbox-->
 +
<div class="floatbox">
 +
<table class="showbox">
 +
      <tr>
 +
              <td>
 +
 
 +
                    <div><a href="https://2013.igem.org/Team:UC_Davis/Project_Overview">
 +
<img src="https://static.igem.org/mediawiki/2013/b/bf/TALpic_UCDavis.jpg" class="blur"></a>
 +
</div>
 +
                    <a href="https://2013.igem.org/Team:UC_Davis/Project_Overview"><h3>Project Overview</h3></a>
 +
                    <p>Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for    inducible repression.
 +
                    </p></a>
 +
 
 +
              </td>
 +
             
 +
<td>
 +
              <a href="https://2013.igem.org/Team:UC_Davis/Data"><img src="https://static.igem.org/mediawiki/2013/d/d5/Resultsicon_UCDavis.jpg" class="blur"></a>
 +
              <a href="https://2013.igem.org/Team:UC_Davis/Data"><h3>Results</h3></a>
 +
                    <p>Check out the cool results of our experiments with RiboTALs. <br />
 +
                            <a href="https://2013.igem.org/Team:UC_Davis/AndersonPromoters"><img src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png" class="checkmark"></a><p>We also made the Anderson promoter family controllable through induction with RiboTALs.</p>
 +
                    </p>
 +
              </td>
 +
                   
 +
             
 +
      </tr>
 +
      <tr>
 +
            <td>
 +
                    <a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview">
 +
<img src="https://static.igem.org/mediawiki/2013/3/35/Humanpracbutton2_UCDavis.jpg" class="blur" />
 +
<!--img src="https://static.igem.org/mediawiki/2013/9/97/UCD_2013_HO_Button.jpg" class="blur"--></a>
 +
                    <a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview"><h3>Human Practices</h3></a>
 +
                    <p>Take a look at how we promote sharing in iGEM through The Depot, an open BioBrick characterization database.<br />
 +
                        <a href="http://dilbert.cs.ucdavis.edu/Depot" class="bold">Visit the Depot!</a>
 +
                    </p>
 +
              </td>
 +
<td>
 +
              <a href="https://2013.igem.org/Team:UC_Davis/Criteria"><img src="https://static.igem.org/mediawiki/2013/f/f3/Judgingbutton_UCDavis.jpg" class="blur"</a>
 +
              <a href="https://2013.igem.org/Team:UC_Davis/Criteria"><h3>Judging Criteria</h3></a>
 +
                    <p>Here's the criteria that we met for this year's team.
 +
                    </p>
 +
              </td>
 +
 
 +
 
 +
           
 +
      </tr>
 +
</table>
 +
 
 +
</div>
 +
<!--End Showbox-->
 +
 
 +
<div class="floatbox">
 +
<h1>Welcome</h1>
 +
<p>
 +
<img src="https://static.igem.org/mediawiki/2013/2/2a/IGEM_2013_Group_Photo.png" width="200" height="300" align="left" class="genpic">
 +
<!--<img src="https://static.igem.org/mediawiki/2013/f/fd/UCDavisMeettheteam.gif" width="200" height="300" align="left" class="genpic">-->
 +
Welcome to the 2013 UC Davis iGEM Wiki!<br></br>We have created a novel class of transcription factors known as RiboTALs. We sought to address the constraints placed on circuit design by the limited number of well characterized promoters at our disposal, and their respective transcription factors. Our device is a hybrid system composed of two parts: Transcriptional Activator-Like (TAL) effectors which can be engineered to bind to and repress any sequence of interest and riboswitches that can respond to any inducer molecule due to their engineerable and modular aptamer binding domains. <br></br> We also designed and implemented a Biobrick characterization database called The Depot to promote sharing and openness in iGEM.</p>
 +
</div>
 +
 
 +
<div id="twitter">
 +
<a class="twitter-timeline" width=220px height=300px href="https://twitter.com/UCDavisiGEM" data-widget-id="363400889542778880" data-theme="dark">
 +
Tweets by @UCDavisiGEM</a>
 +
</div>
 +
 
 +
<div id="sitemapbox" class="floatbox">
 +
</div>
 +
 
 +
 
 +
</body>
 +
<script type="text/javascript">
 +
      $("#sitemapbox").load("https://2013.igem.org/Template:Team:UC_Davis/site_map #sitemap1");
 +
</script>
 +
 
 +
</html>

Latest revision as of 22:43, 5 December 2013

Our Sponsors

Project Overview

Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression.

Results

Check out the cool results of our experiments with RiboTALs.

We also made the Anderson promoter family controllable through induction with RiboTALs.

Human Practices

Take a look at how we promote sharing in iGEM through The Depot, an open BioBrick characterization database.
Visit the Depot!

Judging Criteria

Here's the criteria that we met for this year's team.

Welcome

Welcome to the 2013 UC Davis iGEM Wiki!

We have created a novel class of transcription factors known as RiboTALs. We sought to address the constraints placed on circuit design by the limited number of well characterized promoters at our disposal, and their respective transcription factors. Our device is a hybrid system composed of two parts: Transcriptional Activator-Like (TAL) effectors which can be engineered to bind to and repress any sequence of interest and riboswitches that can respond to any inducer molecule due to their engineerable and modular aptamer binding domains.

We also designed and implemented a Biobrick characterization database called The Depot to promote sharing and openness in iGEM.