Team:USTC CHINA/Notebook/Protocols/Ligation

From 2013.igem.org

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<p><span>Performed with TaKaRa Ligation Kit (Solution I)</span></br>
<p><span>Performed with TaKaRa Ligation Kit (Solution I)</span></br>
1. System</br>
1. System</br>
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<table width="580" border="2">
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Revision as of 17:10, 25 September 2013

Ligation

Performed with TaKaRa Ligation Kit (Solution I)
1. System

Volume (μl)
Solution I 5
DNA (fragment + carrier) 5

fragment : carrier (radio in mol)= 3:1~10:1
carrier≥0.03 pmol
2. Gently vortex the samples and spin down.
3. Incubate at 16°C in a low temperature water thermostat for at least 3h.

notebook

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