Team:USTC CHINA/Parts

From 2013.igem.org

(Difference between revisions)
 
(37 intermediate revisions not shown)
Line 1: Line 1:
 +
{{USTC-China/hidden}}
<html>
<html>
 +
<head>
 +
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/main.css?action=raw&ctype=text/css" />
 +
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:USTC_CHINA/pro.css?action=raw&ctype=text/css" />
<style type="text/css">
<style type="text/css">
-
/* hiding the top section*/
+
.part-col-1{float:left;width:290px;padding:10px;height:200px;}
-
body{position:absolute; top:0px; width:100%; height:2480px;}
+
.part-col-1 h1{line-height:24px;height:40px;}
-
#top-section{
+
.part-col-2{float:left;width:145px;padding:5px;height:280px;}
-
  height:0px;
+
.part-col-2 h1{height:40px;line-height:20px;}
-
    border:none;
+
-
    width:980px;
+
-
    margin:0 auto;
+
-
    padding:0 0 0 0;
+
-
    background-color:transparent;
+
-
    overflow:hide;
+
-
 
+
-
}
+
-
#p-logo{display:none;}
+
-
#search-controls{display:none;}
+
-
#top{display:none;}
+
-
.firstHeading{display:none;}
+
-
/* end of hiding the top section*/
+
-
 
+
-
/* global setting*/
+
-
#globalWrapper{position:absolute; top:0px;left:0px; width:100%; padding:0 0 0
+
-
 
+
-
0;margin:0 0 0 0;height:100%;}
+
-
#content{
+
-
    background-color:transparent;
+
-
    border:none;
+
-
    width:1200px;
+
-
    height:700px;
+
-
    marign:0 auto;
+
-
    padding:0 0 0 0;
+
-
    top:-40px;
+
-
    }
+
-
#bodyContent{
+
-
    background-color:transparent;
+
-
    border:none;
+
-
   
+
-
    }
+
-
siteSub{
+
-
    display:none;
+
-
    border:none;
+
-
    }
+
-
contentSub{
+
-
    display:none;
+
-
    }
+
-
/* end of global setting*/
+
-
 
+
-
/*hiding the footer-box*/
+
-
#footer-box{display:none;}
+
-
#catlinks{display:none;}
+
-
/*end of hiding the footer book*/
+
-
 
+
-
/* menu (page, edit ...) */
+
-
#menubar {
+
-
    background-color:transparent;
+
-
    position: relative;
+
-
    float:left;
+
-
    white-space: nowrap;
+
-
    top:-6px;
+
-
    width: 490px;
+
-
    z-index: 5000;
+
-
    font-family: sans-serif;
+
-
    font-size: 95%;
+
-
    line-height: 1em;
+
-
    z-index:99;
+
-
}
+
-
 
+
-
 
+
-
.left-menu, .left-menu a {
+
-
    text-align: left;
+
-
    color:#999999;
+
-
    text-transform: lowercase;
+
-
}
+
-
 
+
-
.left-menu:hover {
+
-
    color: #D00000;
+
-
    background-color: transparent;
+
-
}
+
-
 
+
-
.right-menu{width:400px; float:right}
+
-
.right-menu, .right-menu a {
+
-
    right: 0px;
+
-
    text-align: right;
+
-
    color: #999999;
+
-
}
+
-
#menubar ul {
+
-
    color: #999999;
+
-
    list-style: none;
+
-
}
+
-
#menubar li {
+
-
    display: inline;
+
-
    position: relative;
+
-
    cursor: pointer;
+
-
    padding-left: 0px;
+
-
    padding-right: 0px;
+
-
}
+
-
.left-menu li a {
+
-
    padding: 0px 10px 0px 0px;
+
-
}
+
-
.left-menu .selected {
+
-
#    color: #999999;
+
-
}
+
-
#.left-menu .selected:hover {
+
-
#    color: #999999;
+
-
#}
+
-
 
+
-
.left-menu:hover a {
+
-
    color: #999999;;
+
-
}
+
-
 
+
-
.left-menu li a:hover {
+
-
    color: #D00000;
+
-
    text-decoration: underline;
+
-
}
+
-
 
+
-
 
+
-
.right-menu li {
+
-
#    background-color: transparent;
+
-
}
+
-
.right-menu li a {
+
-
    padding: 0px 15px 0px 0px;
+
-
    color: #999999;;
+
-
    background-color: transparent;
+
-
}
+
-
.right-menu li a:hover {
+
-
    color: #D00000;
+
-
    text-decoration: underline;
+
-
}
+
-
/* end menu (edit, page ...) */
+
-
 
+
-
/* navigation bar*/
+
-
 
+
-
#navigationblock{background-color:#fafaf8; position:fixed; top:0px;
+
-
 
+
-
width:1200px; height:130px; overflow:hidden; font-family:Calibri, Arial;
+
-
 
+
-
float:left; margin:0px; padding:0px; z-index:1000; border-bottom:1px solid
+
-
 
+
-
#d3d7d8;}
+
-
#navbarlogo{position:absolute; top:0px; left:0px; width:1200px}
+
-
#navigationbar{position:absolute; left:460px; top:38px; font-size:16px; font-
+
-
 
+
-
weight:100; color:black;}
+
-
#navigationbar > li {display:block; list-style-type:none; float:left;
+
-
 
+
-
width:75px; height:30px; text-align:center; }
+
-
 
+
-
.Navbar_Item{background-color:transparent;}
+
-
.Navbar_Item a {color:black; text-decoration:none;}
+
-
.Navbar_Item > ul {display:none; width:900px; height:80px; text-align:center;
+
-
 
+
-
color:black; z-index:1100; }
+
-
.Navbar_Item > ul > li {float:left; list-style:none; text-align:center;
+
-
 
+
-
background-color:transparent; position:relative; top:10px; padding:0 10px;}
+
-
.Navbar_Item:hover{ border-bottom:1px solid #D00000; color:#D00000;
+
-
 
+
-
background-color:#fafaf8;}
+
-
.Navbar_Item:hover > ul{ display:block;}
+
-
.Navbar_Item:hover >a {color:#D00000; background-color:#fafaf8;}
+
-
.Navbar_Item > ul > li:hover {border-bottom:1px solid #D00000; color:#D00000;
+
-
 
+
-
background-color:#fafaf8;}
+
-
.Navbar_Item > ul > li:hover >a {color:#D00000}
+
-
 
+
-
 
+
-
#Home_Sublist{position:relative; top:0px; left:-50px;}
+
-
#Team_Sublist{position:relative; top:0px; left:-110px;}
+
-
#Project_Sublist{position:relative; top:0px; left:-180px;}
+
-
#Model_Sublist{position:relative; top:0px; left:-140px;}
+
-
#DataPage_Sublist{position:relative; top:0px; left:-60px;}
+
-
#Safety_Sublist{position:relative; top:0px; left:-180px;}
+
-
#HumanPractice_Sublist{position:relative; top:0px; left:-360px;}
+
-
 
+
-
#iGEM_logo{position:absolute; top:30px; left:1090px; height:80px;}
+
-
/*end navigation bar*/
+
-
/*Major body*/
+
-
#MajorBody{position:absolute; top:24px; left:0px; width:1200px; height:340px;
+
-
 
+
-
background-color:#f9f9f7;}
+
-
#LeftNavigation{position:fixed; top:130px; float:left; width:200px;
+
-
 
+
-
height:100%; background-color:#313131;z-index:1000;}
+
-
#ProjectListTitle{position:absolute; top:60px; left:20px; color:#ffffff; font-
+
-
 
+
-
family: calibri, arial, helvetica, sans-serif; border-bottom:0px}
+
-
#ProjectList{position:absolute; top:120px; left:5px; color:#ffffff; font-
+
-
 
+
-
family: calibri, arial, helvetica, sans-serif;}
+
-
#ProjectList > li {display:block; list-style-type:none; width:180px;
+
-
 
+
-
height:30px; font-size:20px; text-align:left; background-color:transparent;}
+
-
#ProjectList a {color:#ffffff; text-decoration:none; line-height:30px;}
+
-
 
+
-
 
+
-
 
+
-
 
+
-
#ModelOverviewContainer{position:absolute; top:130px; left:200px; width:1000px;
+
-
 
+
-
height:210px;}
+
-
#ModelOverviewBackground{position:absolute; top:0px; top:0px;}
+
-
#ModelOverviewTitle{position:absolute;  left:0px; top:50px; width:1000px; 
+
-
 
+
-
border-bottom:0px; color:#FFFFFF; font-size:48px; font-family:calibri,Arial,
+
-
 
+
-
Helvetica, sans-serif;line-height:60px; text-align:center;}
+
-
#MoedlOverviewIntroduction{position:absolute; left:60px; top:55px; border-
+
-
 
+
-
bottom:0px; color:#FFFFFF; font-size:25px; font-family:calibri,Arial,
+
-
 
+
-
Helvetica, sans-serif;line-height:60px; text-align:center;}
+
-
#ModelOverviewContent{position:absolute; top:100px; left:60px; width:700px;
+
-
 
+
-
height:400px;color:#FFFFFF;  font-size:18px;font-family:calibri,Arial,
+
-
 
+
-
Helvetica, sans-serif; text-align:justify; line-height:30px;}
+
-
 
+
-
 
+
-
 
+
-
/*Parts Editing Area*/
+
-
#PartsEditingArea{position:absolute; left:200px; top:340px; width:1000px;
+
-
 
+
-
height:3000px; background-color:#ffffff; padding:60px 0;}
+
-
 
+
-
#PartsEditingArea a{color:#5cc33c}
+
-
.SmallBoxs{position:absolute; width:400px; height:300px; background-
+
-
 
+
-
color:#eeeeee;}
+
-
#FavoritePart1{top:150px; left:80px;}
+
-
#FavoritePart2{top:150px; left:520px;}
+
-
#FavoritePart3{top:490px; left:80px;}
+
-
#FavoritePart4{top:490px; left:520px;}
+
-
#FavoritePart5{top:490px; left:80px;}
+
-
#FavoritePart6{top:490px; left:520px;}
+
-
 
+
-
#ImprovedPart{position:absolute;top:890px; left:80px; width:840px;
+
-
 
+
-
height:300px; background-color:#e5e5e5;}
+
-
 
+
-
#OtherPart{position:absolute;top:1290px; left:80px; width:840px; height:1500px;
+
-
 
+
-
background-color:#e5e5e5;}
+
-
#Dataplot{position:absolute;top:2900px; left:80px; width:840px; height:0px;
+
-
 
+
-
background-color:white;}
+
-
 
+
-
 
+
-
#Title1{position: absolute; top:70px; left:80px; width:200px; background-
+
-
 
+
-
color:#5cc33c;font-size:25px; font-family:calibri,Arial, Helvetica, sans-
+
-
 
+
-
serif;line-height:40px; text-align:center; font-weight:bold; font-
+
-
 
+
-
style:Italic;height:60px;color:#ffffff;}
+
-
#Title2{position: absolute; top:810px; left:80px; width:200px; background-
+
-
 
+
-
color:#5cc33c;font-size:25px; font-family:calibri,Arial, Helvetica, sans-
+
-
 
+
-
serif;line-height:40px; text-align:center;font-weight:bold; font-
+
-
 
+
-
style:Italic;height:60px;color:#ffffff;}
+
-
#Title3{position: absolute; top:1210px; left:80px; width:200px; background-
+
-
 
+
-
color:#5cc33c;font-size:25px; font-family:calibri,Arial, Helvetica, sans-
+
-
 
+
-
serif;line-height:40px; text-align:center; font-weight:bold; font-
+
-
 
+
-
style:Italic;height:60px;color:#ffffff;}
+
-
#Title4{position: absolute; top:2810px; left:80px; width:200px; background-
+
-
 
+
-
color:#5cc33c;font-size:25px; font-family:calibri,Arial, Helvetica, sans-
+
-
 
+
-
serif;line-height:40px; text-align:center; font-weight:bold; font-
+
-
 
+
-
style:Italic;height:60px;color:#ffffff;}
+
-
/*End of Parts Editing Area*/
+
-
 
+
-
 
+
-
 
+
-
/*end of Major body*/
+
-
#AcknowledgementBox{ position:absolute; left:0px; top:590px;
+
-
 
+
-
width:1200px;display:none;}
+
-
 
+
</style>
</style>
 +
</head>
 +
<body background="https://static.igem.org/mediawiki/2013/6/62/2013ustc-china_Light_grey_bg.png">
 +
<div class="bar" align="center">
 +
    <div class="container" align="left">
 +
<div id="igemlogo"><a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a></div>
 +
<ul id="nav">
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li>
 +
        <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a>
 +
            <ul class="subs">
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
 +
            </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>
 +
              <ul class="subs">
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li>
 +
                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></li>
 +
              </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a>
 +
              <ul class="subs">
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li>
 +
              </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice">Human Practice</a>
 +
              <ul class="subs">
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity">Activity</a></li>
 +
              </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a>
 +
              <ul class="subs">
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li>
 +
                  <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li>
 +
                  <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li>
 +
              </ul>
 +
        </li>
 +
        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
 +
    </ul>
 +
        <div id="tlogo"><img src="https://static.igem.org/mediawiki/2013/f/f8/2013ustc-china_T-VACCINE.png" width="100%" height="123" />
 +
        </div>
 +
        </div>
 +
        </div>
-
<!--navigationbar-->
+
<div class="content" align="center">
 +
<div class="conbar1">
 +
<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div></div>
 +
<div class="conbar2">
 +
<div class="leftbar" align="left" style="margin-bottom:30px;">
-
+
<div class="part-col-1">
-
</ul>
+
        <h1>TD1, Transdermal peptide</h1>
-
         <a href="https://igem.org/Team_Wikis?year=2013"><img id="iGEM_logo"  
+
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074000">BBa_K1074000</a></h2>
 +
        <p align="center">TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...</p>
 +
</div>
-
src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
+
<div class="part-col-1">
 +
        <h1>Pgrac+RBS+SamyQ+TD1<br>+GFP</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074006">BBa_K1074006</a></h2>
 +
        <p align="center">This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...</p>
</div>
</div>
-
<!--end navigationbar-->
 
-
<!--MajorBody-->
+
<div class="part-col-2">
-
<div id="MajorBody">
+
        <h1>PHT43</h1>
-
    </div>
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
-
    <div id="ModelOverviewContainer">
+
        <p align="center">PHT43 is a <i>E.coli-B.subtilis</i> shuttle vector allowing high-level expression of secreted proteins in <i>B.subtilis</i>... </p>
-
          <img id="ModelOverviewBackground"  
+
</div>
-
src="https://static.igem.org/mediawiki/igem.org/a/a4/Peking2013_Datapage_background.jpg" />
+
<div class="part-col-2">
-
          <h1 id="ModelOverviewTitle">Parts</h1>
+
        <h1>HBsAg</h1>
-
          <h1 id="MoedlOverviewIntroduction"></h1>
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2>  
-
          <p id="ModelOverviewContent"><br/>
+
        <p align="center">HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...</p>
 +
</div>
-
</p>
+
<div class="part-col-2">
-
    </div>
+
        <h1>Ag85b</h1>
-
 
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2>  
-
 
+
        <p align="center">The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin... </p>
-
<!--parts editing area-->
+
</div>
-
<div id="PartsEditingArea">
+
-
 
+
-
    <div id="FavoritePart1" class="SmallBoxs">
+
-
<p id="Favoritecontent1" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px;font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>TD1, Transdermal peptide</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1074000">Main Page-BBa_K1074000</a>
+
-
</br>TD1 is a short synthetic peptide(ACSSSPSKHCG)identified by in vivo phage
+
-
 
+
-
display, facilitated efficient transdermal protein delivery through intact
+
-
 
+
-
skin. Studies suggested that the peptide creates a transient opening in the
+
-
 
+
-
skin barrier to enable macromolecular material to reach systemic circulation
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
    <div id="FavoritePart2" class="SmallBoxs">
+
-
<p id="Favoritecontent2" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px;font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>Pgrac+RBS+SamyQ+TD1+GFP</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1074006">Main Page-BBa_K1074006</a>
+
-
This is the main circuit of our project to allow high expression of target
+
-
 
+
-
protein(antigen,adjuvant).GFP can be substituted by various proteins via a
+
-
 
+
-
modular PCR or standard cut/ligation method.
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
    <div id="FavoritePart3" class="SmallBoxs">
+
-
<p id="Favoritecontent3" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px; font-size:18px;font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>PHT43</b></br>
+
-
PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of
+
-
 
+
-
secreted proteins in B.subtilis. It is based on the strong σA-dependent
+
-
 
+
-
promoter preceding the groE operon of B. subtilis which has been converted into
+
-
 
+
-
an efficiently controllable (IPTGinducible)promoter by addition of the lac
+
-
 
+
-
operator. An efficient Shine-Dalgarno (SD) sequence as well as a multiple
+
-
 
+
-
cloning site (BamH I, Xba I, Aat II, Sma I) were also inserted. To obtain
+
-
 
+
-
secretion of recombinant proteins, the coding region for the signal peptide of
+
-
 
+
-
the amyQ gene encoding an α-amylase was fused to the SD sequence.
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
    <div id="FavoritePart4" class="SmallBoxs">
+
-
<p id="Favoritecontent4" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px; font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>HBsAg</b></br>
+
-
HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates
+
-
 
+
-
current hepatitis B infection.
+
-
Sequence and Features
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
 
+
-
 
+
-
 
+
-
    <div id="ImprovedPart">
+
-
<p id="Favoritecontent1" style="position:absolute; top:20px; right:20px;
+
-
 
+
-
left:20px; font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>NahR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031610">Main Page-
+
-
 
+
-
BBa_K1031610</a></br>
+
-
Pre-existing Part: <a href="http://partsregistry.org/Part:BBa_J61051">Main
+
-
 
+
-
Page-BBa_J61051</a>
+
-
</br>
+
-
BBa_K1031610 is upgraded from previous part BBa_J61051 designed by Arkin Lab.
+
-
NahR is a 34 kDa transcription factor mined from <i>Pseudomonas putida</i>
+
-
 
+
-
which binds to <i>nah</i> and <i>sal</i> promoters to activate transcription in
+
-
 
+
-
response to the inducer salicylate. We constructed a Psal/NahR biosensor
+
-
 
+
-
circuit using sfGFP as reporter gene. Adaptor of this biosensor not only
+
-
 
+
-
improves detection efficiency but also expands detection profile.
+
-
</br>
+
-
<a href="https://2013.igem.org/Team:Peking/Project/BioSensors/NahR">Visit
+
-
 
+
-
Wiki</a>
+
-
</p>
+
-
    </div>
+
-
 
+
-
<div id="OtherPart">
+
-
<p id="Summary1" style="position:relative; left:20px; font-size: 25px; font-
+
-
 
+
-
family:calibri,Arial, Helvetica, sans-serif;line-height:40px;" >
+
-
<b>Parts for Biosensors</b>
+
-
</p>
+
-
<p style="position: relative; top:0px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>DmpR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031221">BBa_K1031221</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031222">BBa_K1031222</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031223">BBa_K1031223</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031224">BBa_K1031224</a> are the
+
-
 
+
-
RBS library of DmpR biosensor's reporter. </br>
+
-
</p>
+
-
<p style="position: relative; top:20px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>HbpR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031300">BBa_K1031300</a> is the
+
-
 
+
-
coding sequence of HbpR with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031301">BBa_K1031301</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031302">BBa_K1031302</a> are the
+
-
 
+
-
RBS library of HbpR biosensor's reporter. </br>
+
-
</p>
+
-
<p style="position: relative; top:40px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>HcaR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031410">BBa_K1031410</a> is the
+
-
 
+
-
coding sequence of HcaR with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031441">BBa_K1031441</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031442">BBa_K1031442</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031443">BBa_K1031443</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031444">BBa_K1031444</a> are the
+
-
 
+
-
Pc library of HbpR to tune its performance . </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031420">BBa_K1031420</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031421">BBa_K1031421</a> are the
+
-
 
+
-
RBS library of HcaR biosensor's reporter. </br>
+
-
 
+
-
</p>
+
-
<p style="position: relative; top:50px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>CapR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031100">BBa_K1031100</a> is the
+
-
 
+
-
coding sequence of CapR transcriptional factor. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031111">BBa_K1031111</a> and     
+
-
 
+
-
   
+
-
<a href="http://partsregistry.org/Part:BBa_K1031112">BBa_K1031112</a> are basic
+
-
 
+
-
sensor construction with different CapR expression level.  </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031113">BBa_K1031113</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031114">BBa_K1031114</a> are
+
-
 
+
-
reporters with different RBS for the CapR biosensor. </br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:60px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>HpaR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031510">BBa_K1031510</a> is the
+
-
 
+
-
coding sequence of HpaR transcriptional factor. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031520">BBa_K1031520</a> and     
+
-
 
+
-
   
+
-
<a href="http://partsregistry.org/Part:BBa_K1031521">BBa_K1031521</a> are
+
-
 
+
-
reporters with different RBS for the HpaR biosensor. </br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:70px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>NahR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031610">BBa_K1031610</a> is the
+
-
 
+
-
NahR biosensor circuit.</br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:80px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>PaaX</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031710">BBa_K1031710</a> is the
+
-
 
+
-
coding sequence of PaaX with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031742">BBa_K1031742</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031743">BBa_K1031743</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031744">BBa_K1031744</a> are the
+
-
 
+
-
Pc library of PaaX. </br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:90px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>XylS</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031911">BBa_K1031911</a> is the
+
-
 
+
-
coding sequence of XylS transcriptional factor with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031912">BBa_K1031912</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031913">BBa_K1031913</a> and     
+
-
 
+
-
   
+
-
<a href="http://partsregistry.org/Part:BBa_K1031916">BBa_K1031916</a> are the
+
-
 
+
-
Pc library of XylS. </br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:100px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>XylR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031803">BBa_K1031803</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031804">BBa_K1031804</a> are the
+
-
 
+
-
RBS library of XylR biosensor's reporter. </br>
+
-
</p>
+
-
 
+
-
 
+
-
<p id="Summary2" style="position:relative; top:120px; left:20px; font-size:
+
-
 
+
-
25px; font-family:calibri,Arial, Helvetica, sans-serif;line-height:40px;" >
+
-
<b>Parts for Adaptors</b>
+
-
 
+
-
<p style="position: relative; top:110px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>NahF</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031620">BBa_K1031620</a> is the
+
-
 
+
-
coding sequence of XylS transcriptional factor with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031621">BBa_K1031621</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031622">BBa_K1031622</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031623">BBa_K1031623</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031624">BBa_K1031624</a> and     
+
-
 
+
-
   
+
-
<a href="http://partsregistry.org/Part:BBa_K1031625">BBa_K1031625</a>are the
+
-
 
+
-
Pc library of NahF. </br>
+
-
</p>
+
-
 
+
-
<p id="Summary3" style="position:relative; top:120px; left:20px; font-size:
+
-
 
+
-
25px; font-family:calibri,Arial, Helvetica, sans-serif;line-height:40px;" >
+
-
<b>Parts for Band-Pass Filter</b>
+
-
<p style="position: relative; top:90px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031021">BBa_K1031021</a> is the
+
-
 
+
-
hybrid promoter for the reporter node, which can be activated as well as
+
-
 
+
-
inhibited. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031011">BBa_K1031011</a> is the
+
-
 
+
-
transcriptional factor that can activate its cognate promoter, which is used as
+
-
 
+
-
the activate node in the band-pass filter. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031013">BBa_K1031013</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031014">BBa_K1031014</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031015">BBa_K1031015</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031016">BBa_K1031016</a> are RBS
+
-
 
+
-
library to tune the expression of C1, under the control of pSal promoter, which
+
-
 
+
-
is a crucial part for band-pass filter. </br>
+
 +
<div class="part-col-2">
 +
        <h1>Protective Antigen Domain 4</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2>
 +
        <p align="center">Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax... </p>
</div>
</div>
-
 
+
<div class="part-col-2">
-
 
+
        <h1>LTB</h1>
-
<div id="Title1">
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>  
-
<p>Favorite Parts</p>
+
        <p align="center">This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of <i>E.coli</i>...</p>
</div>
</div>
-
<div id="Title2">
+
<div class="part-col-2">
-
<p>Improved Parts</p>
+
        <h1>Pctc+RBS+<br>amilCP</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>
 +
        <p align="center">Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in <i>B.subtilis</i> activated by endogenous sigma factor B ...</p>
</div>
</div>
-
<div id="Title3">
+
<div class="part-col-2">
-
<p>Part List</p>
+
        <h1>SdpA,SdpB,<br>SpbC</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>
 +
        <p align="center">Killing factor of <i>B.subtilis</i> ,SpbC Induces the lysis of other <i>B.subtilis</i> cells that have not entered the sporulation pathway...</p>
</div>
</div>
 +
<div class="part-col-2">
 +
        <h1>Pgrac+RBS+SDP</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>
 +
        <p align="center">Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of <i>B.subtilis</i>...</p>
 +
</div>
 +
<div class="clear"></div>
 +
<div class="part-col-2">
 +
        <h1>PsdpRI+RBS<br>+SDP</h1>
 +
      <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>
 +
        <p align="center">We construct this gene circuit as our kill switch to kill the engineered <i>B.subtilis</i> for the safety purpose...</p>
 +
</div>
 +
<div class="part-col-2">
 +
       
 +
        <h1>Pgrac promoter</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074012">BBa_K1074012</a></h2>
 +
        <p align="center">Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG... </p>
</div>
</div>
-
<!--end of parts editing area-->
 
-
+
<div class="part-col-2">
-
</div><!--end of major body-->
+
        <h1>promoter sdpR/I</h1>
-
<img id="AcknowledgementBox"  
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>
 +
        <p align="center">This part is a sigma factor A-dependent promotor of the gene sdpR derived from the <i>B.subtilis</i>...</p>
 +
</div>
 +
<div class="part-col-2">
-
src="https://static.igem.org/mediawiki/igem.org/c/c4/Peking_Sponsors.jpg">
 
-
<!--end of acknowledgement box-->
 
-
<script type="text/javascript"  
+
        <h1>SamyQ</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074014">BBa_K1074014</a></h2>
 +
        <p align="center">SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in <i>B.subtilis</i> WB800N...</p>
 +
</div>
-
src="http://ajax.googleapis.com/ajax/libs/jquery/1.8.0/jquery.min.js"></script>
 
-
<script type="text/javascript">
 
-
$(window).scroll(function(){
 
-
  if(0==document.getElementById("content").offsetLeft){
 
-
$('#navigationblock').css('left',-$(window).scrollLeft());}
 
-
  else{$('#navigationblock').css('left',document.getElementById
 
-
("content").offsetLeft); }
+
<div class="part-col-2">
-
});
+
        <h1>RBS for Pgrac</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>
 +
        <p align="center">An efficient RBS for promoter Pgrac(BBa_K1074012) in <i>B.subtilis</i>.../p>
 +
</div>
-
$(window).resize(function(){
+
<div class="part-col-2">
-
  if(0==document.getElementById("content").offsetLeft){
+
        <h1>RBS for Pctc</h1>
-
 
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>
-
$('#navigationblock').css('left',-$(window).scrollLeft());}
+
        <p align="center">This is an efficient RBS for promoter Pctc(BBa_K143010) in <i>B.subtilis</i>...</p>
-
  else{$('#navigationblock').css('left',document.getElementById
+
</div>
-
 
+
-
("content").offsetLeft); }
+
-
});
+
-
 
+
-
 
+
-
function MoveInSlide(SlideId)
+
-
{
+
-
$(SlideId).animate({top:"0px"});
+
-
         
+
-
};
+
-
 
+
-
function MoveOutSlide(SlideId)
+
-
{
+
-
$(SlideId).animate({top:"280px"});
+
-
       
+
-
};
+
-
 
+
-
 
+
-
</script>
+
 +
<div class="clear"></div>
 +
</div>
 +
<div class="rightbar">
 +
<div class="port-sidebar-border"><h>Project</h></div>
 +
<div class="clear"></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/AdvancingWork">Advancing Work</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/FutureWork">Future Work</a></div>
 +
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
 +
</div></div></div>       
 +
       
 +
</body>
</html>
</html>

Latest revision as of 12:33, 28 October 2013

TD1, Transdermal peptide

BBa_K1074000

TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...

Pgrac+RBS+SamyQ+TD1
+GFP

BBa_K1074006

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...

PHT43

BBa_K1074001

PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis...

HBsAg

BBa_K1074002

HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...

Ag85b

BBa_K1074003

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin...

Protective Antigen Domain 4

BBa_K1074004

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax...

LTB

BBa_K1074005

This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli...

Pctc+RBS+
amilCP

BBa_K1074007

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B.subtilis activated by endogenous sigma factor B ...

SdpA,SdpB,
SpbC

BBa_K1074009

Killing factor of B.subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway...

Pgrac+RBS+SDP

BBa_K1074010

Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of B.subtilis...

PsdpRI+RBS
+SDP

BBa_K1074011

We construct this gene circuit as our kill switch to kill the engineered B.subtilis for the safety purpose...

Pgrac promoter

BBa_K1074012

Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG...

promoter sdpR/I

BBa_K1074013

This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis...

SamyQ

BBa_K1074014

SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in B.subtilis WB800N...

RBS for Pgrac

BBa_K1074015

An efficient RBS for promoter Pgrac(BBa_K1074012) in B.subtilis.../p>

RBS for Pctc

BBa_K1074016

This is an efficient RBS for promoter Pctc(BBa_K143010) in B.subtilis...

Project

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Parts"