Team:USTC CHINA/Parts
From 2013.igem.org
(Difference between revisions)
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<h1>PHT43</h1> | <h1>PHT43</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2> | ||
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- | <h1> | + | <h1>Pctc+RBS+amilCP</h1> |
- | <p> | + | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2> |
+ | <p>Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). amilCP(BBa_K592009), blue chromoprotein,naturally exhibits strong color when expressed. when bacterial grow to the post-log phase, nutrient and space stress arise,Promoter ctc should be activited. We assemble this gene circuit just to expect a visual signal when B.subtilis grow to the post-log phase. </p> | ||
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- | <h1> | + | <h1>SdpA,SdpB,SpbC</h1> |
- | <p> | + | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2> |
+ | <p>Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, being able to bind to SdpI thereby triggering a signal transduction cascade that protects the cell against the toxic effects of its own SdpC. We use this killing factor to build a kill switch for Bacillus subtilis. </p> | ||
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- | <h1> | + | <h1>Pgrac+RBS+SDP</h1> |
- | <p> | + | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2> |
+ | <p>Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis. We put the SDP operon under the strong Regulated promoter Pgrac(BBa_K1074012) to see whether overexpression of spbc can induce the suicide of engineered Bacillus subtilis. </p> | ||
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- | <h1> | + | <h1>PsdpRI+RBS+SDP</h1> |
- | <p> | + | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2> |
+ | <p>We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.Killing is mediated by the exported toxic protein SpbC. Extracellular SpbC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR. </p> | ||
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Revision as of 13:52, 27 September 2013