Team:USTC CHINA/Parts
From 2013.igem.org
(Difference between revisions)
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<h1>PHT43</h1> | <h1>PHT43</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2> | ||
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<h1>HBsAg</h1> | <h1>HBsAg</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2> | ||
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<h1>Ag85b</h1> | <h1>Ag85b</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2> | ||
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<h1>Protective Antigen Domain 4</h1> | <h1>Protective Antigen Domain 4</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2> | ||
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<h1>LTB</h1> | <h1>LTB</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2> | ||
<p>This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli.However,in vitro,like Chinese Hamster Ovarylera toxin B subunit (CTB), it is an efficient mucosal adjuvant and carrier molecule for the generation of mucosal antibody responses and induction of systemic T-cell tolerance to linked antigens. So we use it to enhance the immune response to our transdermal vaccine. </p> | <p>This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli.However,in vitro,like Chinese Hamster Ovarylera toxin B subunit (CTB), it is an efficient mucosal adjuvant and carrier molecule for the generation of mucosal antibody responses and induction of systemic T-cell tolerance to linked antigens. So we use it to enhance the immune response to our transdermal vaccine. </p> | ||
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<h1>Pctc+RBS+amilCP</h1> | <h1>Pctc+RBS+amilCP</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2> | ||
<p>Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). amilCP(BBa_K592009), blue chromoprotein,naturally exhibits strong color when expressed. when bacterial grow to the post-log phase, nutrient and space stress arise,Promoter ctc should be activited. We assemble this gene circuit just to expect a visual signal when B.subtilis grow to the post-log phase. </p> | <p>Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). amilCP(BBa_K592009), blue chromoprotein,naturally exhibits strong color when expressed. when bacterial grow to the post-log phase, nutrient and space stress arise,Promoter ctc should be activited. We assemble this gene circuit just to expect a visual signal when B.subtilis grow to the post-log phase. </p> | ||
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<h1>SdpA,SdpB,SpbC</h1> | <h1>SdpA,SdpB,SpbC</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2> | ||
<p>Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, being able to bind to SdpI thereby triggering a signal transduction cascade that protects the cell against the toxic effects of its own SdpC. We use this killing factor to build a kill switch for Bacillus subtilis. </p> | <p>Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, being able to bind to SdpI thereby triggering a signal transduction cascade that protects the cell against the toxic effects of its own SdpC. We use this killing factor to build a kill switch for Bacillus subtilis. </p> | ||
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<h1>Pgrac+RBS+SDP</h1> | <h1>Pgrac+RBS+SDP</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2> | ||
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<h1>PsdpRI+RBS+SDP</h1> | <h1>PsdpRI+RBS+SDP</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2> | ||
<p>We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.Killing is mediated by the exported toxic protein SpbC. Extracellular SpbC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR. </p> | <p>We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.Killing is mediated by the exported toxic protein SpbC. Extracellular SpbC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR. </p> | ||
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<h1>promoter sdpR/I</h1> | <h1>promoter sdpR/I</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2> | ||
<p>This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis. It's activated as the toxin sdpC existed,while the natural pructrue of the promotor, sdpR/I ,confers immunity to the SdpC toxin. </p> | <p>This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis. It's activated as the toxin sdpC existed,while the natural pructrue of the promotor, sdpR/I ,confers immunity to the SdpC toxin. </p> | ||
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<h1>RBS for Pgrac</h1> | <h1>RBS for Pgrac</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2> | ||
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<h1>RBS for Pctc</h1> | <h1>RBS for Pctc</h1> | ||
<h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2> | <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2> |
Revision as of 14:02, 27 September 2013