TD1, Transdermal peptide

BBa_K1074000

TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin. Studies suggested that the peptide creates a transient opening in the skin barrier to enable macromolecular material to reach systemic circulation.

Pgrac+RBS+SamyQ+TD1
+GFP

BBa_K1074006

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method.

PHT43

BBa_K1074001

PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis.

HBsAg

BBa_K1074002

HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection.

Ag85b

BBa_K1074003

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs).

Protective Antigen Domain 4

BBa_K1074004

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax.

LTB

BBa_K1074005

This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli.However,in vitro,like Chinese Hamster Ovarylera toxin B subunit (CTB), it is an efficient mucosal adjuvant and carrier molecule for the generation of mucosal antibody responses and induction of systemic T-cell tolerance to linked antigens. So we use it to enhance the immune response to our transdermal vaccine.

Overview

In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries.

Overview

In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries.

Overview

In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries.

Overview

In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries.

Overview

In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries.

Overview

In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries.

Overview

In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries.