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Team:Bielefeld-Germany/Labjournal/June
From 2013.igem.org
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*Isolation of genomic DNA from S. oneidensis | *Isolation of genomic DNA from S. oneidensis | ||
</div> | </div> | ||
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<div class="cyt_genomiso"> | <div class="cyt_genomiso"> | ||
| - | + | **4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78 | |
| - | *4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78 | + | **4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67 |
| - | *4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67 | + | |
**Dilution to PCR template: 4-2006-453: 5.5ng/µl | **Dilution to PCR template: 4-2006-453: 5.5ng/µl | ||
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</div> | </div> | ||
<div class="cyt_amp_mtr_hl"> | <div class="cyt_amp_mtr_hl"> | ||
| - | + | *Amplification of the mtrCAB cluster | |
</div> | </div> | ||
<div class="cyt_amp_mtr"> | <div class="cyt_amp_mtr"> | ||
| - | < | + | *Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9 |
| + | *Clear Bands at the expected 5.2kb | ||
| + | </div> | ||
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| + | <div class="cyt_clean_mtr_hl"> | ||
| + | *PCR-CleanUp | ||
</div> | </div> | ||
| - | |||
| - | |||
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<div class="cyt_clean_mtr"> | <div class="cyt_clean_mtr"> | ||
| - | + | **PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement | |
| - | PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement | + | ***Lane2: 4-2106-451: 7.4 ng/µl |
| - | + | ***Lane5: 4-2106-451: 8.5 ng/µl | |
| - | + | ||
| - | + | ||
| - | + | ||
</div> | </div> | ||
Revision as of 08:04, 25 September 2013
June
Milesstones
1.Week
Organization
- iGEM-Team Bielefeld will support the ‘CeBiTec Pupil Academy’ from 26.-30. August with an own experiment.
MFC
Mediators
Cytochromes
Biosafety
Porines
2.Week
Organization
- Thanks to NEB Biolabs for the free iGEM kit with many useful laboratory things for all German iGEM teams.
- We are working on our first press release.
- Having a short radio contribution in the Bielefeld university campus radio (radio 87.9 hertz).
MFC
Mediators
Cytochromes
Biosafety
Porines
3.Week
Organization
- Nothing organizational, empty, empty, empty space.
MFC
Mediators
Cytochromes
- Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C
- Isolation of genomic DNA from S. oneidensis
- 4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78
- 4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67
- Dilution to PCR template: 4-2006-453: 5.5ng/µl
- Amplification of the mtrCAB cluster
- Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
- Clear Bands at the expected 5.2kb
- PCR-CleanUp
- PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement
- Lane2: 4-2106-451: 7.4 ng/µl
- Lane5: 4-2106-451: 8.5 ng/µl
- PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement
Biosafety
Porines
<a name=4.Week>4.Week</a>
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/#">Organization</a>
- Let’s go to Lyon, flights are booked for the European jamboree in Lyon from 11.-13. October 2013.
- We will participate at ‘BioNRW pHD Student Convention’ in Düsseldorf at 13. July.
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC">MFC</a>
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Mediators">Mediators</a>
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Cytochromes">Cytochromes</a>
<a href="https://2013.igem.org/Team:Bielefeld-Germany/BioSafety">Biosafety</a>
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Porins">Porines</a>
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