Team:Bielefeld-Germany/Labjournal/June

From 2013.igem.org

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Revision as of 09:47, 27 September 2013




June

Milesstones





1.Week

Organization

  • iGEM-Team Bielefeld will support the ‘CeBiTec Pupil Academy’ from 26.-30. August with an own experiment.

MFC

Mediators

Cytochromes

Biosafety

Porines









2.Week

Organization

  • Thanks to NEB Biolabs for the free iGEM kit with many useful laboratory things for all German iGEM teams.
  • We are working on our first press release.
  • Having a short radio contribution in the Bielefeld university campus radio (radio 87.9 hertz).


MFC

Mediators

Cytochromes

Biosafety

Porines












3.Week

Organization

  • Nothing organizational, empty, empty, empty space.

MFC

Mediators

Cytochromes

  • Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C


  • Isolation of genomic DNA from S. oneidensis
    • 4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78
    • 4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67
    • Dilution to PCR template: 4-2006-453: 5.5ng/µl


  • Amplification of the mtrCAB cluster
    • Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
    • Clear Bands at the expected 5.2kb


  • PCR-CleanUp
    • PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement
    • Lane2: 4-2106-451: 7.4 ng/µl
    • Lane5: 4-2106-451: 8.5 ng/µl


Biosafety

Porines











4.Week

Organization

  • Let’s go to Lyon, flights are booked for the European jamboree in Lyon from 11.-13. October 2013.
  • We will participate at ‘BioNRW pHD Student Convention’ in Düsseldorf at 13. July.

MFC

Mediators

Cytochromes

Biosafety

Porines












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