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Team:Bielefeld-Germany/Labjournal/October
From 2013.igem.org
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====Porines==== | ====Porines==== | ||
| + | *Great results for'' Escherichia coli'' KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to ''Escherichia coli'' KRX Wildtyp with Microbial Fuel Cell cultivation. | ||
| + | *According to our assumptions, the extracellular electron transfer mediated by electron shuttles is improved in the OprF strain resulting in an increased bioelectricity output. (Figure 1 and 2) | ||
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| + | [[Image:IGEM_Bielefeld_Voltage_OprF_Genetic.jpg|300px|thumb|left|<p align="justify"> '''Figure 1: Microbial Fuel Cell results from cultivation of ''Escherichia coli'' KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to ''Escherichia coli'' KRX Wildtyp. Voltage curve from ''Escherichia coli'' KRX Wildtyp, ''Escherichia coli'' KRX with OprF (<bbpart>BBa_K1172502</bbpart>), M9 medium with used mediator New Methylene Blue and M9 medium without mediator is shown over time. '''</p>]] | ||
| + | [[Image:IGEM_Bielefeld_ElectricCharge_OprF_Genetic.jpg|300px|thumb|center|<p align="justify"> '''Figure 2: Microbial Fuel Cell results from cultivation of ''Escherichia coli'' KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to ''Escherichia coli'' KRX Wildtyp. Electric charge curve from ''Escherichia coli'' KRX Wildtyp and ''Escherichia coli'' KRX with OprF (<bbpart>BBa_K1172502</bbpart>). M9 medium was used with mediator New Methylene Blue. '''</p>]] | ||
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| + | *''Escherichia coli'' KRX with OprF (<bbpart>BBa_K1172502</bbpart>) shows 100 % higher voltage and electric charge than ''E. coli Wildtyp''. | ||
Revision as of 10:57, 2 October 2013
October
Milestones
Week 22
Organization
MFC
Mediators
Cytochromes
Biosafety
Porines
- Great results for Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to Escherichia coli KRX Wildtyp with Microbial Fuel Cell cultivation.
- According to our assumptions, the extracellular electron transfer mediated by electron shuttles is improved in the OprF strain resulting in an increased bioelectricity output. (Figure 1 and 2)
Figure 1: Microbial Fuel Cell results from cultivation of Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to Escherichia coli KRX Wildtyp. Voltage curve from Escherichia coli KRX Wildtyp, Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>), M9 medium with used mediator New Methylene Blue and M9 medium without mediator is shown over time.
Figure 2: Microbial Fuel Cell results from cultivation of Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>) in contrast to Escherichia coli KRX Wildtyp. Electric charge curve from Escherichia coli KRX Wildtyp and Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>). M9 medium was used with mediator New Methylene Blue.
- Escherichia coli KRX with OprF (<bbpart>BBa_K1172502</bbpart>) shows 100 % higher voltage and electric charge than E. coli Wildtyp.
Week 23
Organization
MFC
Mediators
Cytochromes
Biosafety
Porines
Week 24
Organization
MFC
Mediators
Cytochromes
Biosafety
Porines
Week 25
Organization
MFC
Mediators
Cytochromes
Biosafety
Porines
Contents |
