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Team:Bielefeld-Germany/Labjournal/June
From 2013.igem.org
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<h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Cytochromes">Cytochromes</a></h4> | <h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Cytochromes">Cytochromes</a></h4> | ||
Revision as of 12:33, 2 September 2013
June
Milesstones
1.Week
MFC
Mediators
Cytochromes
Biosafety
Porines
2.Week
MFC
Mediators
Cytochromes
Biosafety
Porines
3.Week
MFC
Mediators
Cytochromes
Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C
Isolation of genomic DNA from S. oneidensis
4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78
4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67
Dilution to PCR template: 4-2006-453: 5.5ng/µl
Amplification of the mtrCAB cluster with gradient PhusionPCR
Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
Clear Bands at the expected 5.2kb
PCR-CleanUp of lane 2 and 5 with the Machery-Nagel-PCR-CleanUp-Kit
Lane2: 4-2106-451: 7.4 ng/µl
Lane5: 4-2106-451: 8.5 ng/µl
