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Team:TU-Delft/Notebook/2013/07/10/
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Latest revision as of 08:00, 4 October 2013
Notebook
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10th of July
Lab work
1. Made glycerol stocks for all the transformations done on 9/7/13.
2. DNA extraction of all transformations to send for sequencing.
3. Experiment with tetracycline for checking of pTET:lysis device failed.
4. Started new transformations :
| Part | Resistance | Cells |
| pTET:lysis device | Ampicillin | DH5Alpha |
| AgrB:AgrD | Kanamycin | BL21 |
| pConst | Ampicillin | DH5Alpha |
All transformations were streaked on agar plates with corresponding antibiotic resistance.
5. Prepared flasks for autoclaving.
