In the last months we were able to ...
- ... construct a catalytically inactive version of Cas9 and thus generate a DNA binding protein.
- ... combine this modified dCas9 with different transcriptional effectors.
- ... express this fusion proteins in various mammalian cell lines.
- ... control mammalian gene expression via our modified CRISPR/Cas fusion proteins.
- ... build devices for controling gene expression by light stimulus.
- ... provide an RNA plasmid for easily inserting sequences for crRNAs which target every desired target.
- ... build an online tool that generates customized manuals for using our toolkit
- ... develop a method to assess the DNA binding capacity of our dCas9-fusion proteins.
- ... make our dCas9 accessible to the whole iGEM community by mutating illegal iGEM restriction sites.
- ... In summary, we can now offer a universally applicable toolkit for gene regulation.