Team:Freiburg/Highlights/2

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Revision as of 10:54, 4 October 2013

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HIGHLIGHTS

In the last months we were able to ...
- ... construct a catalytically inactive version of Cas9 and thus generate a DNA binding protein.
- ... combine this modified dCas9 with different transcriptional effectors.
- ... express this fusion proteins in various mammalian cell lines.
- ... control mammalian gene expression via our modified CRISPR/Cas fusion proteins.
- ... build devices for controling gene expression by light stimulus.
- ... provide an RNA plasmid for easily inserting sequences for crRNAs which target every desired target.
- ... build an online tool that generates customized manuals for using our toolkit
- ... develop a method to assess the DNA binding capacity of our dCas9-fusion proteins.
- ... make our dCas9 accessible to the whole iGEM community by mutating illegal iGEM restriction sites.
- ... In summary, we can now offer a universally applicable toolkit for gene regulation.
Activation

Click here to read more about our activation project
The Toolbox

Click here to read more about what the toolbox is and how you can use it.

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