Team:Bielefeld-Germany/Labjournal/May
From 2013.igem.org
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*Initiating lab work on the sub-project endogenous mediator [[Team:Bielefeld-Germany/Project/GldA|Glycerol dehydrogenase]]. | *Initiating lab work on the sub-project endogenous mediator [[Team:Bielefeld-Germany/Project/GldA|Glycerol dehydrogenase]]. | ||
- | *Starting lab work with the first successful PCR: gldA with pre- and suffix overlaps could be amplified from ''Escherichia coli'' genome. | + | *Starting lab work with the first successful PCR: ''gldA'' with pre- and suffix overlaps could be amplified from ''Escherichia coli'' genome. |
*The main work however is still planning of our sub-projects, designing experiments and a lot of research. | *The main work however is still planning of our sub-projects, designing experiments and a lot of research. | ||
*Finding sponsors goes ahead. Many companies like our project and want to support us. | *Finding sponsors goes ahead. Many companies like our project and want to support us. |
Revision as of 01:57, 5 October 2013
May
Milestones
- Initiating lab work on the sub-project endogenous mediator Glycerol dehydrogenase.
- Starting lab work with the first successful PCR: gldA with pre- and suffix overlaps could be amplified from Escherichia coli genome.
- The main work however is still planning of our sub-projects, designing experiments and a lot of research.
- Finding sponsors goes ahead. Many companies like our project and want to support us.
Week 1
Organization
- We were already able to find sponsors for our team: IIT BIEKUBA, IIT Biotech, Evonik, PlasmidFactory and FisherScientific will support us.
MFC
Mediators
- Glycerol dehydrogenase
- Primerdesign for isolation of gldA gene from Escherichia coli KRX strain, with overlaps for BioBrick prefix and suffix:
- Forward primer gldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC
- Reverse primer gldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG
2.Week
Organization
- Distribution of our team in various subgroups for best work efficiency.
- We’ve organized a barbecue to thank the working group of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory.
MFC
3.Week
Organization
- Planning the trip to the European jamboree in Lyon from October 11th to 13th, 2013.
MFC
Mediators
- Glycerol dehydrogenase
- Starting first cultivation of Escherichia coli KRX strain for complete genome isolation.
- Successful genome isolation of Escherichia coli KRX.
- Successful PCR on the gldA gene of Escherichia coli KRX strain.
- The gldA PCR product was isolated by agarose gel electrophoresis and purified.
- Bands are on at the expected size of 1050 bp.
4.Week
Organization
- We will also take part at the congress ‘‘Next generation of biotechnological processes 2020+‘‘ in Berlin on June 27th, 2013.
- Having a second presentation at Merck KGaA head office in Darmstadt for explaining our project in detail. We are happy that Merck will be our next supporter in 2013.
MFC