Team:Bielefeld-Germany/Labjournal/August

The backbone and shipping vector psB1C3 was cut with EcoRI, SpeI, the insert ccmAH with EcoRI and XbaI to perfom a standard suffix insertion.
We used approx. 200ng insert and 42ng vector, which repressnts a 2-fold molar excess of insert.
The Ligation was incubated for 15 min at 37°C, followed by a heat-inactivation at 80°C for 20 min.
The subsequent gel electrophoresis did not yield any positiv results, probably due to the use of buffer and enzymes from two different manufactures.