September

Milestones

Week 18

Organization

• This week, we supported the CeBiTec pupil academy with supervising the ‘Synthetic Biology’ experiment. In addition we had a presentation about iGEM and the Bielefeld iGEM projects.
• Our team assigned the Track Food & Energy in this year, with the project title ‘Ecolectricity – currently available’. Project abstract, safety forms and team roster were added.

MFC

Mediators

Cytochromes

Biosafety

Porines

• Successful Hexadecan-Assay for characterization of hydrophobicity of the outer membrane. Using Escherichia coli KRX Wildtyp and comparing it with:
  • <partinfo>BBa_K1172502</partinfo>: <partinfo>BBa_K1172501</partinfo> + <partinfo>BBa_K525998</partinfo>
  • <partinfo>BBa_K1172503</partinfo>: <partinfo>BBa_K1172501</partinfo> + <partinfo>BBa_J04500</partinfo>
  • <partinfo>BBa_K1172504</partinfo>: <partinfo>BBa_K1172501</partinfo> + <partinfo>BBa_K608007</partinfo>
  • <partinfo>BBa_K1172505</partinfo>: <partinfo>BBa_K1172501</partinfo> + <partinfo>BBa_K608006</partinfo>
  • <partinfo>BBa_K1172507</partinfo>: <partinfo>BBa_K1172501</partinfo> + <partinfo>BBa_K608002</partinfo>

Table 3: Results of the Hexadecane-Hydrophobicity-Assay. Comparison of protein Hydrophobicity between Escherichia coli KRX wild type and Escherichia coli KRX with <partinfo>BBa_K1172502</partinfo>, <partinfo>BBa_K1172503</partinfo>, <partinfo>BBa_K1172504</partinfo>, <partinfo>BBa_K1172505</partinfo> and <partinfo>BBa_K1172507</partinfo>. Affinity to hexadecane (Hydrophobicity) with standard deviation and enhancement in comparison to the wild-type is shown

Figure 5: Results of the Hexadecane-Hydrophobicity-Assay. Comparison of protein Hydrophobicity between Escherichia coli KRX wild type and Escherichia coli KRX with <partinfo>BBa_K1172502</partinfo>, <partinfo>BBa_K1172503</partinfo>, <partinfo>BBa_K1172504</partinfo>, <partinfo>BBa_K1172505</partinfo> and <partinfo>BBa_K1172507</partinfo>. Affinity to hexadecane (Hydrophobicity) with standard deviation and enhancement in comparison to the wild-type is shown

• The hydrophobicity increases continuously with increasing promoter strength up to 221% hydrophobizity in contrast to wildtyp strain.

Week 19

Organization

• Our WDR TV contribution could be seen in the ‘OWL local time’ and in the WDR library.
• We have successfully performed the day of synthetic biology in the city of Bielefeld. We were able to explain many people our project with the help of our poster and the kids had a lot of fun with performing different experiments for introducing them into scientific work. DNA isolation from fruit and vegetables, pipetting of bright colors, chromatography with markers, a potato battery and microscopy were on the program. All in all a very successful day.

MFC

Mediators

Cytochromes

Biosafety

Porines

Week 20

Organization

• iGEM-Team Bielefeld 2013 T-shirts and hoodies were designed and are in production. Orange shirts with a great Ecolectricity logo. We are pleased to wear them in Lyon.
• As announced, we have participated at the conference '60 Years of DNA’ in Berlin at 14. September and received good reviews for our project.

MFC

Mediators

Cytochromes

Biosafety

Porines

Week 21

Organization

MFC

Mediators

Cytochromes

Biosafety

Porines