Team:Bielefeld-Germany/Labjournal/May

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Revision as of 01:57, 5 October 2013 by Nadiya (Talk | contribs)



May


Milestones

  • Initiating lab work on the sub-project endogenous mediator Glycerol dehydrogenase.
  • Starting lab work with the first successful PCR: gldA with pre- and suffix overlaps could be amplified from Escherichia coli genome.
  • The main work however is still planning of our sub-projects, designing experiments and a lot of research.
  • Finding sponsors goes ahead. Many companies like our project and want to support us.






Week 1

Organization

  • We were already able to find sponsors for our team: IIT BIEKUBA, IIT Biotech, Evonik, PlasmidFactory and FisherScientific will support us.


MFC

Mediators

  • Glycerol dehydrogenase
    • Primerdesign for isolation of gldA gene from Escherichia coli KRX strain, with overlaps for BioBrick prefix and suffix:
    • Forward primer gldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC
    • Reverse primer gldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG










2.Week

Organization

  • Distribution of our team in various subgroups for best work efficiency.
  • We’ve organized a barbecue to thank the working group of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory.


MFC









3.Week

Organization

  • Planning the trip to the European jamboree in Lyon from October 11th to 13th, 2013.


MFC

Mediators

  • Glycerol dehydrogenase


Table 1: Standard phusion PCR master mix.

Table 2: Two step standard phusion PCR program for gldA amplification.


    • The gldA PCR product was isolated by agarose gel electrophoresis and purified.
    • Bands are on at the expected size of 1050 bp.


Figure 1: Agarose gel from PCR on the gldA gene of Escherichia coli KRX strain with forward and reverse primer gldA. As a ladder we used [http://www.thermoscientificbio.com/nucleic-acid-electrophoresis/generuler-1-kb-dna-ladder-ready-to-use-250-to-10000-bp GeneRuler™ 1 kb DNA Ladder from Thermo Scientific].










4.Week

Organization

  • We will also take part at the congress ‘‘Next generation of biotechnological processes 2020+‘‘ in Berlin on June 27th, 2013.
  • Having a second presentation at Merck KGaA head office in Darmstadt for explaining our project in detail. We are happy that Merck will be our next supporter in 2013.


MFC











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