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Motivation
Biosafety is an essential aspect when taking part in iGEM especially when you work with living organisms which could possibly get out of your application by damage or incorrect handling. In order to counter this problem there exist useful systems to prevent the bacteria from escaping or killing the bacteria when they are outside of the application. To complement this archive we constructed not only one system but also three systems which differ in leakiness and strength.
For this approach we combined two common Biosafety-ideas, an auxotrophy and a toxic gene product, in one device. So the constructed Biosafety-System takes the best of this two approaches and is characterized by a double kill-swtich system, which provides additional a higher plasmid stability and a higher resistance towards undesirable mutations. In one sentence: Our Biosafety-System is save!
