Team:Bielefeld-Germany/Biosafety/Biosafety System

From 2013.igem.org

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==Overview==
==Overview==
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[[Image:Bielefeld-germany-project-overview-nanowires.png|left|thumb|250px|'''Figure 1:''' Principle of electron transfer from bacteria to anode via nanowires.]]
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[[Image:IGEM Bielefeld 2913 Biosafety E.coli bewaffnet save ohne hintergrund.jpg|250px|left|thumb]]
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Multiple bacteria form special, highly conductive pili, which are required for survival in anaerobe environment. Electrons, which are generated through the oxidation of different substrates, can be transported by these pili and transferred to alternative electron-acceptors, such as sulfur or iron. These so called nanowires could furthermore increase the number of bacteria contacting the surface of the anode. These properties make them another interesting option, regarding the optimization of E. coli for the use in MFCs. Because several large gene clusters are required to form nanowires in organisms such as ''Geobacter sulfurreducens'', it will be first investigated, whether the existing type 4 pili are suitable for electron conduction by ''E. coli'' as well.  
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Biosafety is an essential aspect when taking part in iGEM especially when you work with living organisms which could possibly get out of your application by damage or incorrect handling. In order to counter this problem there exist useful systems to prevent the bacteria from escaping or killing the bacteria when they are outside of the application. To complement this archive we constructed not only one system but also three systems which differ in leakiness and strength.
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For this approach we combined two common Biosafety-ideas, an auxotrophy and a toxic gene product, in one device. So the constructed Biosafety-System takes the best of this two approaches and is characterized by a double kill-swtich system. This double kill-switch mechanism provides additional a higher plasmid stability and a higher resistance towards undesirable mutations. In one sentence: Our Biosafety-System is save!
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==Theory==
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==TetOR Alive==
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==Genetic Approach==
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==Lac of Growth==
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==Results==
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==AraCtive==

Revision as of 22:05, 29 September 2013



Biosafety System


Overview

IGEM Bielefeld 2913 Biosafety E.coli bewaffnet save ohne hintergrund.jpg

Biosafety is an essential aspect when taking part in iGEM especially when you work with living organisms which could possibly get out of your application by damage or incorrect handling. In order to counter this problem there exist useful systems to prevent the bacteria from escaping or killing the bacteria when they are outside of the application. To complement this archive we constructed not only one system but also three systems which differ in leakiness and strength. For this approach we combined two common Biosafety-ideas, an auxotrophy and a toxic gene product, in one device. So the constructed Biosafety-System takes the best of this two approaches and is characterized by a double kill-swtich system. This double kill-switch mechanism provides additional a higher plasmid stability and a higher resistance towards undesirable mutations. In one sentence: Our Biosafety-System is save!











TetOR Alive

Lac of Growth

AraCtive

References







Contents

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