Team:Bielefeld-Germany/Labjournal/Analytics

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<div id=globalwrapper style="padding-left:20px; padding-right:20px;">
<h1 style="color:#ff6600;">Analytics</h1>
<h1 style="color:#ff6600;">Analytics</h1>
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<a name="analytics"><span style="color:#ff6600; padding-left=22px">[Analytics]</span></a>
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<a name="analytics"><span style="color:#ff6600; padding-left=22px">[SDS PAGE]</span></a>
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<br><b><u> subtitle</u></b>
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<a name="tecan"><span style="color:#ff6600; padding-left=22px">[Fluorescence measurements]</span></a>
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<br><b><u>Fluorescence measurements </u></b>
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Measuring of mRFP with Tecan Infinite® M200 platereader
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Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination
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Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark
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To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination)
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Measure the fluorescence in a platereader (we used a Tecan Infinite® M200 platereader) with following settings:
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20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm)
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Measurement mode: Top
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Excitation: 584 nm
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Emission: 620 nm
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Number of reads: 25
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Manual gain: 100
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Integration time: 20 µs
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<br><b><u>subtitle</u></b>
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Revision as of 19:42, 25 September 2013





Analytics

[SDS PAGE]

subtitle
[Fluorescence measurements]

Fluorescence measurements Measuring of mRFP with Tecan Infinite® M200 platereader Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination) Measure the fluorescence in a platereader (we used a Tecan Infinite® M200 platereader) with following settings: 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm) Measurement mode: Top Excitation: 584 nm Emission: 620 nm Number of reads: 25 Manual gain: 100 Integration time: 20 µs

subtitle
[title]



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