Team:Bielefeld-Germany/Labjournal/Analytics
From 2013.igem.org
(Difference between revisions)
m |
m |
||
Line 38: | Line 38: | ||
<br><br> | <br><br> | ||
<h1 style="color:#ff6600;">Analytics</h1> | <h1 style="color:#ff6600;">Analytics</h1> | ||
+ | <br> | ||
<div class="sds_headline"> | <div class="sds_headline"> | ||
<a name="sds"><span style="color:#ff6600; padding-left=22px">[SDS PAGE]</span></a> | <a name="sds"><span style="color:#ff6600; padding-left=22px">[SDS PAGE]</span></a> |
Revision as of 19:47, 25 September 2013
Analytics
subtitle
Fluorescence measurements Measuring of mRFP with Tecan Infinite® M200 platereader Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination) Measure the fluorescence in a platereader (we used a Tecan Infinite® M200 platereader) with following settings: 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm) Measurement mode: Top Excitation: 584 nm Emission: 620 nm Number of reads: 25 Manual gain: 100 Integration time: 20 µs
subtitle