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Team:Bielefeld-Germany/Labjournal/Cultivation
From 2013.igem.org
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<h1>Cultivation</h1> | <h1>Cultivation</h1> | ||
<div class="media_1_headline"> | <div class="media_1_headline"> | ||
| - | <a name="media_1"><span style="color:#ff6600">[Media] | + | <a name="media_1"><span style="color:#ff6600">[Media]</span></a> |
</div> | </div> | ||
<div class="media_1"> | <div class="media_1"> | ||
| - | <br><u>Chloramphenicol stock solution</u> | + | <br><b><u> Chloramphenicol stock solution</u></b> |
<p>-Solubilize 20 mg mL-1 Chloramphenicol in 100 % Ethanol </p> | <p>-Solubilize 20 mg mL-1 Chloramphenicol in 100 % Ethanol </p> | ||
<p>-Store at -20 °C</p> | <p>-Store at -20 °C</p> | ||
| - | < | + | <br><b><u> DNA loading buffer</u></b> |
| + | <p> - 50 % (v/v) glycerol </p> | ||
| + | <p> - 1 mM EDTA </p> | ||
| + | <p> - 0.1 % (w/v) bromphenol blue </p> | ||
| + | <p> - Solve in ddH2O</p> | ||
| + | <br><b><u> LB medium</u></b> | ||
| + | <p> - 10 g Trypton </p> | ||
| + | <p> - 5 g yeast extract </p> | ||
| + | <p> - 10 g NaCl </p> | ||
| + | <p><i> - 12 g Agar-Agar (for plates) </p></i> | ||
| + | <p> - Adjust pH to 7.4</p> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <br><b><u>TAE buffer</u></b> | ||
| + | <p>For 1 L of 50 x TAE buffer you need:</p> | ||
| + | <p> - 242.48 g Tris </p> | ||
| + | <p> - 41.02 g Sodiumacetate</p> | ||
| + | <p> - 18.612 g EDTA </p> | ||
| + | <p> - Adjust pH to 7.8 with acetic acid </p> | ||
| + | <p> - Solve in dH2O </p> | ||
| + | |||
| + | <p><i>10 mL of the stock is diluted in 1 L dH2O for the gel electrophoresis (0.5 x TAE buffer) </i></p> | ||
| + | |||
| + | |||
| + | <!-- | ||
| + | <br><b><u></u></b> | ||
| + | <p> -</p> | ||
--> | --> | ||
<br><br><br><br><br><br><br><br><br><br><br><br><br> | <br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
Revision as of 15:46, 4 September 2013
Cultivation
Chloramphenicol stock solution
-Solubilize 20 mg mL-1 Chloramphenicol in 100 % Ethanol
-Store at -20 °C
DNA loading buffer
- 50 % (v/v) glycerol
- 1 mM EDTA
- 0.1 % (w/v) bromphenol blue
- Solve in ddH2O
LB medium
- 10 g Trypton
- 5 g yeast extract
- 10 g NaCl
- 12 g Agar-Agar (for plates)
- Adjust pH to 7.4
TAE buffer
For 1 L of 50 x TAE buffer you need:
- 242.48 g Tris
- 41.02 g Sodiumacetate
- 18.612 g EDTA
- Adjust pH to 7.8 with acetic acid
- Solve in dH2O
10 mL of the stock is diluted in 1 L dH2O for the gel electrophoresis (0.5 x TAE buffer)
