Team:Bielefeld-Germany/Labjournal/May

From 2013.igem.org

(Difference between revisions)
Line 110: Line 110:
<br><br><br><br>
<br><br><br><br>
</div>
</div>
 +
 +
 +
 +
<html>
 +
<div id="nav2" style="width:210px; height:40px; margin-left:752px; margin-top:-80px; float:right; position:fixed; padding-left:15px;">
 +
 +
<div class="navbutton" id="home" style="float:left; padding-left:10px; margin-left:10px; padding-top:0px;">
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany" title="Jump to Frontpage">
 +
<img src="https://static.igem.org/mediawiki/2013/f/f6/Bielefeld-Germany2013-ButtonHome.png" height="15px">
 +
</a>
 +
</div>
 +
<div class="navbutton" id="top" style="float:left; padding-left:10px; margin-left:10px; padding-top:0px;">
 +
<a href="#" title="Jump to top">
 +
<img src="https://static.igem.org/mediawiki/2013/a/ab/Bielefeld-Germany2013-Up_orange_new.png" height="15px">
 +
</a>
 +
</div>
 +
<div class="navbutton" id="left" style="float:left; padding-left:10px; margin-left:10px; padding-bottom:0px;">
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal/Prologue" title="Jump to previous month">
 +
<img src="https://static.igem.org/mediawiki/2013/2/2e/Bielefeld-Germany2013-left_orange.png" height="12px">
 +
</a>
 +
</div>
 +
<div class="navbutton" id="right" style="float:left; padding-left:10px; margin-left:10px; padding-bottom:0px;">
 +
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Labjournal/June" title="Jump to next month">
 +
<img src="https://static.igem.org/mediawiki/2013/1/1b/Bielefeld-Germany2013-right_orange.png" height="12px">
 +
</a>
 +
</div>
 +
</div>
 +
</html>
<div id="rightcol" style="width:210px; height:450px; margin-left:752px; margin-top:-60px; float:right; position:fixed; overflow-y:scroll;    box-shadow:0px 0px 2px 0px grey;" padding:0px 20px;>
<div id="rightcol" style="width:210px; height:450px; margin-left:752px; margin-top:-60px; float:right; position:fixed; overflow-y:scroll;    box-shadow:0px 0px 2px 0px grey;" padding:0px 20px;>

Revision as of 13:40, 2 October 2013



May


Milestones

  • Starting lab work with the first successful PCR: GldA with Pre- and Suffix overlaps could be amplified from Escherichia coli genome.






Week 1

Organization

  • We were already able to find sponsors for our team: IIT BIEKUBA, IIT Biotech, Evonik, PlasmidFactory and FisherScientific will support us.


MFC

Mediators

  • Glycerol dehydrogenase
    • Primerdesign for isolation of GldA gene from Escherichia coli KRX strain, with overlaps for Biobrick Prefix and Suffix:
    • Forward Primer GldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC
    • Reverse Primer GldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG










2.Week

Organization

  • Distribution of our team in various subgroups for best work efficiency.
  • We’ve organized a working group barbecue to thank the AG of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory.


MFC









3.Week

Organization

  • Planning the trip to the European jamboree in Lyon from 11.-13. October 2013.


MFC

Mediators

  • Glycerol dehydrogenase


Table 1: Standard Phusion PCR Master Mix.

Table 2: Two step standard Phusion PCR programm for GldA amplification.


    • GldA PCR product was isolated by Agarose gel electrophorese and purificated.
    • Bands are at expected size of 1050 bp.


Figure 1: Agarosegel from PCR on the GldA gene of Escherichia coli KRX strain with forward and reverse primer GldA. For Ladder we used GeneRuler™ 1 kb DNA Ladder fromThermo Scientific.










4.Week

Organization

  • We will also take part at the congress "Next generation of biotechnological processes 2020+" in Berlin at 27. June 2013.
  • Having a second presentation at Merck KGaA head office in Darmstadt for explaining our project in detail. We are happy that Merck will be our next supporter in 2013.


MFC










Contents

Retrieved from "http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/May"