May

Milestones

• Starting lab work with the first successful PCR: GldA with Pre- and Suffix overlaps could be amplified from Escherichia coli genome.

Week 1

Organization

• We were already able to find sponsors for our team: IIT BIEKUBA, IIT Biotech, Evonik, PlasmidFactory and FisherScientific will support us.

MFC

Mediators

• Glycerol dehydrogenase
  • Primerdesign for isolation of GldA gene from Escherichia coli KRX strain, with overlaps for Biobrick Prefix and Suffix:
  • Forward Primer GldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC
  • Reverse Primer GldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG

2.Week

Organization

• Distribution of our team in various subgroups for best work efficiency.
• We’ve organized a working group barbecue to thank the AG of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory.

MFC

3.Week

Organization

• Planning the trip to the European jamboree in Lyon from 11.-13. October 2013.

MFC

Mediators

• Glycerol dehydrogenase
  • Starting first cultivation of Escherichia coli KRX strain for complete genome isolation.
  • Successful genome isolation of Escherichia coli KRX.
  • Successful PCR on the GldA gene of Escherichia coli KRX strain.

Table 1: Standard Phusion PCR Master Mix.

Table 2: Two step standard Phusion PCR programm for GldA amplification.

• • GldA PCR product was isolated by Agarose gel electrophorese and purificated.
  • Bands are at expected size of 1050 bp.

Figure 1: Agarosegel from PCR on the GldA gene of Escherichia coli KRX strain with forward and reverse primer GldA. For Ladder we used GeneRuler™ 1 kb DNA Ladder fromThermo Scientific.

4.Week

Organization

• We will also take part at the congress "Next generation of biotechnological processes 2020+" in Berlin at 27. June 2013.
• Having a second presentation at Merck KGaA head office in Darmstadt for explaining our project in detail. We are happy that Merck will be our next supporter in 2013.

MFC