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Team:Bielefeld-Germany/Labjournal/May
From 2013.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
| - | *Initiating | + | *Initiating lab work on the sub-project endogenous mediator [[Team:Bielefeld-Germany/Project/GldA|Glycerol dehydrogenase]]. |
*Starting lab work with the first successful PCR: GldA with Pre- and Suffix overlaps could be amplified from ''Escherichia coli'' genome. | *Starting lab work with the first successful PCR: GldA with Pre- and Suffix overlaps could be amplified from ''Escherichia coli'' genome. | ||
| - | *The main work however is still planning of our | + | *The main work however is still planning of our subprojects, designing experiments and a lot of research. |
*Finding sponsors goes ahead. Many companies like our project and want to support us. | *Finding sponsors goes ahead. Many companies like our project and want to support us. | ||
<br><br><br><br> | <br><br><br><br> | ||
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====Mediators==== | ====Mediators==== | ||
*Glycerol dehydrogenase | *Glycerol dehydrogenase | ||
| - | **Primerdesign for isolation of GldA gene from ''Escherichia coli'' KRX strain, with overlaps for | + | **Primerdesign for isolation of GldA gene from ''Escherichia coli'' KRX strain, with overlaps for BioBrick Prefix and Suffix: |
**Forward Primer GldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC | **Forward Primer GldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC | ||
**Reverse Primer GldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG | **Reverse Primer GldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG | ||
| Line 48: | Line 48: | ||
====Organization==== | ====Organization==== | ||
*Distribution of our team in various subgroups for best work efficiency. | *Distribution of our team in various subgroups for best work efficiency. | ||
| - | *We’ve organized a | + | *We’ve organized a barbecue to thank the working group of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory. |
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====Organization==== | ====Organization==== | ||
| - | *Planning the trip to the European jamboree in Lyon from 11. | + | *Planning the trip to the European jamboree in Lyon from October 11. to 13. 2013. |
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[[Image:IGEM_Bielefeld_Standard_Phusion_PCR_Master_MixLRO.jpg|300px|thumb|left| <p align="justify">'''Table 1: Standard Phusion PCR Master Mix. '''</p>]] | [[Image:IGEM_Bielefeld_Standard_Phusion_PCR_Master_MixLRO.jpg|300px|thumb|left| <p align="justify">'''Table 1: Standard Phusion PCR Master Mix. '''</p>]] | ||
| - | [[Image:IGEM_Bielefeld_Standard_Phu_PCR_GldA_OprF.jpg|300px|thumb|center| <p align="justify">'''Table 2: Two step standard Phusion PCR | + | [[Image:IGEM_Bielefeld_Standard_Phu_PCR_GldA_OprF.jpg|300px|thumb|center| <p align="justify">'''Table 2: Two step standard Phusion PCR program for GldA amplification. '''</p>]] |
| - | **GldA PCR product was isolated by | + | **GldA PCR product was isolated by agarose gel electrophoresis and [[Team:Bielefeld-Germany/Labjournal/Molecular#Used Kits| purified]]. |
| - | **Bands are at expected size of 1050 bp. | + | **Bands are on at the expected size of 1050 bp. |
| - | [[Image:IGEM_Bielefeld_GldA_PCR_Gel.jpg|200px|thumb|left| <p align="justify">'''Figure 1: | + | [[Image:IGEM_Bielefeld_GldA_PCR_Gel.jpg|200px|thumb|left| <p align="justify">'''Figure 1: Agarose gel from PCR on the GldA gene of ''Escherichia coli'' KRX strain with forward and reverse primer GldA. As a ladder we used [http://www.thermoscientificbio.com/nucleic-acid-electrophoresis/generuler-1-kb-dna-ladder-ready-to-use-250-to-10000-bp GeneRuler™ 1 kb DNA Ladder from Thermo Scientific]. '''</p>]] |
Revision as of 21:37, 4 October 2013
May
Milestones
- Initiating lab work on the sub-project endogenous mediator Glycerol dehydrogenase.
- Starting lab work with the first successful PCR: GldA with Pre- and Suffix overlaps could be amplified from Escherichia coli genome.
- The main work however is still planning of our subprojects, designing experiments and a lot of research.
- Finding sponsors goes ahead. Many companies like our project and want to support us.
Week 1
Organization
- We were already able to find sponsors for our team: IIT BIEKUBA, IIT Biotech, Evonik, PlasmidFactory and FisherScientific will support us.
MFC
Mediators
- Glycerol dehydrogenase
- Primerdesign for isolation of GldA gene from Escherichia coli KRX strain, with overlaps for BioBrick Prefix and Suffix:
- Forward Primer GldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC
- Reverse Primer GldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG
2.Week
Organization
- Distribution of our team in various subgroups for best work efficiency.
- We’ve organized a barbecue to thank the working group of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory.
MFC
3.Week
Organization
- Planning the trip to the European jamboree in Lyon from October 11. to 13. 2013.
MFC
Mediators
- Glycerol dehydrogenase
- Starting first cultivation of Escherichia coli KRX strain for complete genome isolation.
- Successful genome isolation of Escherichia coli KRX.
- Successful PCR on the GldA gene of Escherichia coli KRX strain.
Table 1: Standard Phusion PCR Master Mix.
Table 2: Two step standard Phusion PCR program for GldA amplification.
- GldA PCR product was isolated by agarose gel electrophoresis and purified.
- Bands are on at the expected size of 1050 bp.
Figure 1: Agarose gel from PCR on the GldA gene of Escherichia coli KRX strain with forward and reverse primer GldA. As a ladder we used GeneRuler™ 1 kb DNA Ladder from Thermo Scientific.
4.Week
Organization
- We will also take part at the congress "Next generation of biotechnological processes 2020+" in Berlin at 27. June 2013.
- Having a second presentation at Merck KGaA head office in Darmstadt for explaining our project in detail. We are happy that Merck will be our next supporter in 2013.
MFC
