Team:Calgary/Project/DataPage

From 2013.igem.org

Revision as of 03:07, 28 September 2013 by Emily Hicks (Talk | contribs)

Data Page

Characterization of new parts submitted to the Registry

  • Submitted two new reporters to the registry: Beta-lactamase (Bba_ K1189008) and Recombinant Human Ferritin (Bba_ K1189008) . We submitted these parts alone, with 6-his tags and lacI inducible promoters and fused to TALE-A (Slovenia 2012).
  • Expressed Ferritin (Bba_ K1189008)), which possesses 24 subunits, and showed successful chemical conversion of our expressed protein into Prussian blue ferritin. We also showed that this part could feasibly be used as a reporter in a nitrocellulose test-strip system.
  • Expressed and purified beta lactamase both with and without TALE-A (Bba_ K1189007) and (Bba_ K1189031) and characterized the functionality of these Biobricks as a reporter using both pH and ampicillin resistance assays.

Further characterization and improvement of parts already in the registry