Team:Freiburg/Highlights/2

From 2013.igem.org

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                      <img src="https://static.igem.org/mediawiki/2013/6/6c/Repression.gif" id="gif1" width="5%" alt="play animation" />
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HIGHLIGHTS
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                                Repression
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<br>
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                              <p><a href="https://2013.igem.org/Team:Freiburg/Project/3">
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                              Click here to read more</a> about our repression project
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<p id="h4"><font size="5"><i style="margin-left:95px;">In the last months we were able to ...</i></font></p>
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<ul style="font-size:18px; margin-left:110px;">
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    <li> ... construct a catalytically inactive version of <b>Cas9</b> and thus generate a <b>DNA binding protein</b>.</li>
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    <li> ... combine this modified dCas9 with different transcriptional <b>effectors</b>.</li>
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    <li> ... express this fusion proteins in various <b>mammalian</b> cell lines.</li>
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    <li> ... <b>control</b> mammalian gene expression via our modified CRISPR/Cas fusion proteins.</li>
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    <li> ... build devices for controling gene expression by <b>light stimulus</b>.</li>
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<li> ... provide an RNA plasmid for easily inserting sequences for crRNAs which target every desired target.</li>
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<li> ... build an online tool that generates customized <b>manuals</b> for using our toolkit</li>
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    <li> ... develop a method to assess the <b>DNA binding capacity</b> of our dCas9-fusion proteins.</li>
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    <li>... make our dCas9 <b>accessible</b> to the whole iGEM community by mutating illegal iGEM restriction sites</b>.</li>
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<li><i> ... In summary, we can now offer a universally applicable <b>toolkit</b> for gene regulation.</i></li>
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Revision as of 10:37, 4 October 2013


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  • HIGHLIGHTS


    In the last months we were able to ...

    • ... construct a catalytically inactive version of Cas9 and thus generate a DNA binding protein.
    • ... combine this modified dCas9 with different transcriptional effectors.
    • ... express this fusion proteins in various mammalian cell lines.
    • ... control mammalian gene expression via our modified CRISPR/Cas fusion proteins.
    • ... build devices for controling gene expression by light stimulus.
    • ... provide an RNA plasmid for easily inserting sequences for crRNAs which target every desired target.
    • ... build an online tool that generates customized manuals for using our toolkit
    • ... develop a method to assess the DNA binding capacity of our dCas9-fusion proteins.
    • ... make our dCas9 accessible to the whole iGEM community by mutating illegal iGEM restriction sites.
    • ... In summary, we can now offer a universally applicable toolkit for gene regulation.
  • Activation

    Click here to read more about our activation project

  • The Toolbox

    Click here to read more about what the toolbox is and how you can use it.

    • Retrieved from "http://2013.igem.org/Team:Freiburg/Highlights/2"