Team:Freiburg/Project/Coop

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To live the spirit of iGEM our team collaborated with other teams.

Team Heidelberg

When we introduced Gibson cloning in our team we were facing quite some problems but after several months of optimizing protocol and reagents and doing refinements we succeeded easily to have positive colonies carrying correctly assembled plasmids. We wanted to pass this laborious achievement and knowledge on to other iGEM teams. To help team Heidelberg establishing Gibson cloning for their project we decided to visit their laboratory.
For team Heidelberg it was very important to get Gibson cloning running as soon as possible. Their constructs are quite complex and time was running short. To demonstrate Gibson cloning we brought along a reporter plasmid of our project. This consists of four parts: the backbone pBS1C3, a CMV promoter, an avGFP and a bGH terminator. Together we calculated the amounts of DNA fragments needed the Gibson reaction and pipetted the mix. After revealing some tricks and myths the Heidelberg iGEMs were able to yield E. coli carrying the reporter plasmid. To verify the functionality of the construct they checked for fluorescence and were happy to see green color.
To top off this collaboration Team Heidelberg provided us with a special cancer cell line, which we could manipulate by CRISPR Cas9.
Besides spending time in their iGEM lab we were shown around on campus and in the city of Heidelberg. Finally we all enjoyed a fabulous dinner in the heart of the historic city center.

Team Norwich

To help the iGEM team of Norwich we also cooperated with this team. Therefore we collected soil in the garden of one of our team members. We sent 15 g soil to NRP-UEA-Norwich, which they want to screen for novel antimycins.
By sending them probes we hope to support this team to successfully conduct their iGEM project.