Team:TMU-Tokyo

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Revision as of 01:07, 25 September 2013 by Masashiohara (Talk | contribs)



Project description


In the iGEM, most teams have inserted their Biobrick parts or devices into the plasmids and use them. It is true that there are many good points to use plasmids, for instance, it is easy to do transformation, and it is convenient to use high copy plasmid when you want to get high gene expression amount. However, there are some bad points to use plasmids too. For example, when the reproduction starting point of plural plasmids are covered, it can’t put in E.coli at the same time. Also it is difficult to control genetic expression closely.

Therefore, in this year, our team tried to standardize a new method to inserted Biobrick parts or devices in a genome of E.coli and used them. Also we really inserted the device which we designed in a genome of E.coli according to our method and functionalized it.



Standardization

In order to insert Biobricks in to a genome of E.coli and functionalize them, we made a new and interesting method and standardize it. In this new method named “NEWS”, we constructed all of our parts by over rap extension PCR and inserted these PCR products in to a genome of E.coli by lambda bacteriophage recombination system, ”RED”. Also we designed a new parts which to improve the present designated vector. With this parts, it can be easily to do cloning the parts which made by PCR.

Additionally, we standardized this method so that other iGEMers could use it. So we’ll apply for New Standard prize.

Implementation ―Genomic Pythagorean Device-

According to the system that we spoke in the right, we really inserted the device named “Genomic Pythagorean Device”, which we designed into a genome of E.coli. “Pythagorean Device” is very famous in Japan because Japanese famous educational TV program "Pythagorean Switch" introduces various Pythagorean Devices. They are known as "Rube Goldberg machines" in the US. Pythagorean Devices are deliberately over-engineered or overdone systems that perform very simple task in very complicated process, usually including some chain reactions. We constructed “Pythagorean Device” in E.coli genome with lambda phage recombination system "RED"


 
  • What is our goal?
  • Why is there a need for our project?
  • Why do we insert parts in a genome?
  • What Biobrick parts did we design ?
  • Which parts did we submit ?
  • Did our parts work ?
  • What kind of application is imaginable ?
  • What is necessary to improve our project ?
  • Are there any relation with our project ?
  • What did we do for securing of safety ?
  • What did we achieve in our project ?


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