Team:TMU-Tokyo/Team attribution

From 2013.igem.org



Our University

　

All members of our team belong to department of Biological Science at Tokyo Metropolitan University, which located in Hachioji city.

　

Address：〒192-0364 1-1, Minamiosawa, Hachioji-city, Tokyo.



TMU-Tokyo official team profile is here.






Support from the following laboratories

The all of following laboratories are in our university.

  • Molecular Genetics Lab
  • Environmental Microbiology Lab
  • Plant Photoregulation Lab
  • Cellular Genetics Lab

    • These laboratories provided the experiment space, some biological materials, and reagent to us. Also some members of these laboratories including our team’s instructors held the safety class for us.





    Materials we have received from the following laboratories

  • Escherichia coli MG1655 red (Km)
  • Escherichia coli MG1655 recA⁻(Tc) (PCR template: tetracycline resistance gene)
  • Bacteriophage P1 (for P1 transduction)
  • pFTGT (PCR template: FLP recombinase)
  • m(C2-Ap)Sm (PCR template : streptomycin resistance gene)
  • pBAD/Myc-HisA (PCR templat : pBAD promoter)
  • pSG76C (PCR template: FRT and chloramphenicol resistance gene)
  • pSG76A2 ((PCR template: FRT and ampicillin resistance gene)
  • pACYC177 (PCR template: kanamycin resistance gene)
  • PCR primers (sequences are not written for IPR Protection)
  • Cm-NR-stu
  • Cm-CR-stu
  • CmC
  • araC2
  • pBAD-C3v
  • TcN
  • TcC3
  • 141-70
  • R6Kori-Xho-3R
  • pACYC177
  • araCHC
  • araC
  • CmNR-Not
  • CmC2

    • From Junichi Kato (Professor, Molecular Genetics Laboratory, Department of Biological Sciences, Tokyo Metropolitan University)


  • pKK009 (pilV gene+shfflon+rci recombinase, vector: pBR322)
  • pKK024 (rci recom　binase, vector: pUC9)
  • pKK040 (shufflon A segment, vector: pUC9)
  • pKK041 (shufflon B segment, vector: pUC9)
  • pKK042 (shufflon C segment, vector: pUC9)
  • 239pUC ( modified shufflon, symmetry recombination site, vector: pUC19)
  • pINV202 (modified shufflon, symmetry inverted recombination site, vector: pUC118 )
  • pDEL302 (modified shufflon, symmetry direct deletion, vector: pUC118)
  • pAG105 (shufflon, vector: pUC18)

    • From Atsuko Gyohda (visiting researcher, Plant Hormone Mechanism Laboratory, Department of Biological Sciences, Tokyo Metropolitan University) & Nobuhisa Furuya (Assistant professor, Molecular Genetics Laboratory, Department of Biological Sciences, Tokyo Metropolitan University)


  • pBluescriptⅡ　SK（+）
  • UAST-Cre

    • From Taro Kaneuchi (Research Assistant, Cellular Genetics Laboratory, Department of Biological Sciences, Tokyo Metropolitan University)




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