Team:USTC CHINA/Project/Results
From 2013.igem.org
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<h1>Stage One:Basic Experiments</h1> | <h1>Stage One:Basic Experiments</h1> | ||
<h2>Introduction</h2> | <h2>Introduction</h2> | ||
- | <p align="justify">As the construction and concentration of recombinant plasmid rely heavily on E.coli system in current molecule experiment protocols of Bacillus subtilis, | + | <p align="justify">As the construction and concentration of recombinant plasmid rely heavily on E.coli system in current molecule experiment protocols of Bacillus subtilis, B.subtilis acts only as the secretory expression vector. Therefore, to verify the practicality of our locus and the transdermal function of recombinant transdermal protein on top of its original functions, we conducted basic experiments on E.coli to verify our assumptions.</p> |
<p align="justify">The following figure shows the stages of our basic experiments:</p> | <p align="justify">The following figure shows the stages of our basic experiments:</p> | ||
<div align="center"><img src="https://static.igem.org/mediawiki/2013/d/d0/Basal_phase.png" width="300" height="400"/></div> | <div align="center"><img src="https://static.igem.org/mediawiki/2013/d/d0/Basal_phase.png" width="300" height="400"/></div> | ||
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<div class="basic-bar"> | <div class="basic-bar"> | ||
<h3 style="font-size:24px;line-height:20px;">3. Verifying the Antigenicity of TD1-Antigen by ELISA</h3> | <h3 style="font-size:24px;line-height:20px;">3. Verifying the Antigenicity of TD1-Antigen by ELISA</h3> | ||
- | <p align="justify"> The antigenicity of the TD1-antigen(fusion protein)strongly proved our theoretical basis. We have set 3 different negative control groups(GFP sample, HBsAg transdermal result, | + | <p align="justify"> The antigenicity of the TD1-antigen(fusion protein)strongly proved our theoretical basis. We have set 3 different negative control groups(GFP sample, HBsAg transdermal result, normal saline) and one positive group standard HBsAg sample.</p> |
<img src="https://static.igem.org/mediawiki/2013/8/85/%E6%97%A0%E6%A0%87%E9%A2%98%2C%2C%2C%2C%2C.png" width="580" height="150"/> | <img src="https://static.igem.org/mediawiki/2013/8/85/%E6%97%A0%E6%A0%87%E9%A2%98%2C%2C%2C%2C%2C.png" width="580" height="150"/> | ||
<div class="atfigure" align="center" style="width:">Fig 4. Verifying the antigenicity of TD1-HBsAg by ELISA.</div> | <div class="atfigure" align="center" style="width:">Fig 4. Verifying the antigenicity of TD1-HBsAg by ELISA.</div> |
Revision as of 13:25, 24 October 2013